Laser-ultrasonic delivery of agents into articular cartilage

Heikki J. Nieminen*, Goncalo Barreto, Mikko A. Finnila, Alejandro Garcia-Perez, Ari Salmi, Sanjeev Ranjan, Kari K. Eklund, Kenneth P. H. Pritzker, Simo Saarakkala, Edward Haeggstrom

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)

Abstract

Research is ongoing to develop drug therapies to manage osteoarthritis (OA) and articular cartilage (AC) injuries. However, means to deliver drug to localized AC lesions are highly limited and not clinically available. This study investigates the capability of laser ultrasound (laser-induced plasma sound source) to deliver agents (methylene blue, MB, in PBS) into bovine AC. Treatment samples (n = 10) were immersed in MB solution simultaneously with LU exposure, while adjacent control 1 tissue (n = 10) was pre-treated with LU followed by immersion in MB and adjacent control 2 tissue (n = 10) was only immersed in MB. AC exposed (n = 22) or not exposed (n = 27) to LU were characterized for anomalies in structure, composition, viability or RNA expression. Optically detected MB content was significantly (p <0.01) higher in treatment samples up to a depth of 500 mu m from AC surface as compared to controls. No major unwanted short-term effects on AC structure, proteoglycan or collagen contents, chondrocyte viability or RNA expression levels were detected. In conclusion, LU can deliver agents into AC without major short-term concerns on safety. LU could reveal new strategies for the development of localized drug therapies in AC.

Original languageEnglish
Article number3991
Pages (from-to)1-12
Number of pages12
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 21 Jun 2017
MoE publication typeA1 Journal article-refereed

Keywords

  • INTENSITY ULTRASOUND
  • DRUG-DELIVERY
  • KNEE OSTEOARTHRITIS
  • JOINT
  • GLYCOSAMINOGLYCANS
  • OVEREXPRESSION
  • VIABILITY
  • SECTIONS

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