Abstract
A laccase from the thermophilic fungus Melanocarpus albomyces was shown to bind to softwood and pure microcrystalline cellulose. The binding isotherm fitted well the Langmuir type one-site binding model. The adsorption parameters indicated that M. albomyces laccase binds with high affinity to cellulose with a relatively low maximum binding capacity, as compared to the values for various cellulases. The binding was shown to be reversible and not influenced by non-specific protein or 0.1-0.5 M Na2SO4. No binding was detected with laccases from Trametes hirsuta or Mauginiella sp., which suggests that binding to cellulose is typical for only some laccases.
Original language | English |
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Pages (from-to) | 251-255 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 576 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - 8 Oct 2004 |
MoE publication type | A1 Journal article-refereed |
Keywords
- ABTS, 2,2-azinobis-(3-ethylbenzthiazoline-6- sulphonate)
- Bacterial microcrystalline cellulose
- BMCC, bacterial microcrystalline cellulose
- BSA, bovine serum albumin
- Cellulose binding
- Melanocarpus albomyces laccase