Laccase from Melanocarpus albomyces binds effectively to cellulose

Laura Leena Kiiskinen, Hetti Palonen, Markus Linder, Liisa Viikari, Kristiina Kruus*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

22 Citations (Scopus)

Abstract

A laccase from the thermophilic fungus Melanocarpus albomyces was shown to bind to softwood and pure microcrystalline cellulose. The binding isotherm fitted well the Langmuir type one-site binding model. The adsorption parameters indicated that M. albomyces laccase binds with high affinity to cellulose with a relatively low maximum binding capacity, as compared to the values for various cellulases. The binding was shown to be reversible and not influenced by non-specific protein or 0.1-0.5 M Na2SO4. No binding was detected with laccases from Trametes hirsuta or Mauginiella sp., which suggests that binding to cellulose is typical for only some laccases.

Original languageEnglish
Pages (from-to)251-255
Number of pages5
JournalFEBS Letters
Volume576
Issue number1-2
DOIs
Publication statusPublished - 8 Oct 2004
MoE publication typeA1 Journal article-refereed

Keywords

  • ABTS, 2,2-azinobis-(3-ethylbenzthiazoline-6- sulphonate)
  • Bacterial microcrystalline cellulose
  • BMCC, bacterial microcrystalline cellulose
  • BSA, bovine serum albumin
  • Cellulose binding
  • Melanocarpus albomyces laccase

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