A laccase from the thermophilic fungus Melanocarpus albomyces was shown to bind to softwood and pure microcrystalline cellulose. The binding isotherm fitted well the Langmuir type one-site binding model. The adsorption parameters indicated that M. albomyces laccase binds with high affinity to cellulose with a relatively low maximum binding capacity, as compared to the values for various cellulases. The binding was shown to be reversible and not influenced by non-specific protein or 0.1-0.5 M Na2SO4. No binding was detected with laccases from Trametes hirsuta or Mauginiella sp., which suggests that binding to cellulose is typical for only some laccases.
- ABTS, 2,2-azinobis-(3-ethylbenzthiazoline-6- sulphonate)
- Bacterial microcrystalline cellulose
- BMCC, bacterial microcrystalline cellulose
- BSA, bovine serum albumin
- Cellulose binding
- Melanocarpus albomyces laccase