KORRIGAN1 and its Aspen Homolog PttCel9A1 Decrease Cellulose Crystallinity in Arabidopsis Stems

Junko Takahashi; Ulla J. Rudsander; Mattias Hedenstrom; Alicja Banasiak; Jesper Harholt; Nicolas Amelot; Peter Immerzeel; Peter Ryden; Satoshi Endo; Farid M. Ibatullin; Harry Brumer; Elena del Campillo; Emma R. Master; Henrik Vibe Scheller; Bjorn Sundberg; Tuula T. Teeri; Ewa J. Mellerowicz

doi:10.1093/pcp/pcp062

KORRIGAN1 and its Aspen Homolog PttCel9A1 Decrease Cellulose Crystallinity in Arabidopsis Stems

Junko Takahashi, Ulla J. Rudsander, Mattias Hedenstrom, Alicja Banasiak, Jesper Harholt, Nicolas Amelot, Peter Immerzeel, Peter Ryden, Satoshi Endo, Farid M. Ibatullin, Harry Brumer, Elena del Campillo, Emma R. Master, Henrik Vibe Scheller, Bjorn Sundberg, Tuula T. Teeri, Ewa J. Mellerowicz^*

^*Corresponding author for this work

Not published at Aalto University

Research output: Contribution to journal › Article › Scientific › peer-review

Abstract

KORRIGAN1 (KOR1) is a membrane-bound cellulase implicated in cellulose biosynthesis. PttCel9A1 from hybrid aspen (Populus tremula L. tremuloides Michx.) has high sequence similarity to KOR1 and we demonstrate here that it complements kor1-1 mutants, indicating that it is a KOR1 ortholog. We investigated the function of PttCel9A1/KOR1 in Arabidopsis secondary growth using transgenic lines expressing 35S::PttCel9A1 and the KOR1 mutant line irx2-2. The presence of elevated levels of PttCel9A1/KOR1 in secondary walls of 35S::PttCel9A1 lines was confirmed by in muro visualization of cellulase activity. Compared with the wild type, 35S::PttCel9A1 lines had higher trifluoroacetic acid (TFA)-hydrolyzable glucan contents, similar Updegraff cellulose contents and lower cellulose crystallinity indices, as determined by (13)C solid-state nuclear magnetic resonance (NMR) spectroscopy. irx2-2 mutants had wild-type TFA-hydrolyzable glucan contents, but reduced Updegraff cellulose contents and higher than wild-type cellulose crystallinity indices. The data support the hypothesis that PttCel9A1/KOR1 activity is present in cell walls, where it facilitates cellulose biosynthesis in a way that increases the amount of non-crystalline cellulose.

Original language	English
Pages (from-to)	1099-1115
Number of pages	17
Journal	PLANT AND CELL PHYSIOLOGY
Volume	50
Issue number	6
DOIs	https://doi.org/10.1093/pcp/pcp062
Publication status	Published - Jun 2009
MoE publication type	A1 Journal article-refereed

Keywords

Aspen
Cellulase
Cellulose
Cell wall
Populus
Wood formation
CARBOHYDRATE-ACTIVE ENZYMES
SECONDARY CELL-WALL
HIGHER-PLANTS
C-13 NMR
WOOD FORMATION
BIOSYNTHESIS
EXPRESSION
THALIANA
ENDO-1,4-BETA-GLUCANASE
GENES

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10.1093/pcp/pcp062

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Takahashi, J., Rudsander, U. J., Hedenstrom, M., Banasiak, A., Harholt, J., Amelot, N., Immerzeel, P., Ryden, P., Endo, S., Ibatullin, F. M., Brumer, H., del Campillo, E., Master, E. R., Scheller, H. V., Sundberg, B., Teeri, T. T., & Mellerowicz, E. J. (2009). KORRIGAN1 and its Aspen Homolog PttCel9A1 Decrease Cellulose Crystallinity in Arabidopsis Stems. PLANT AND CELL PHYSIOLOGY, 50(6), 1099-1115. https://doi.org/10.1093/pcp/pcp062

Takahashi, Junko ; Rudsander, Ulla J. ; Hedenstrom, Mattias ; Banasiak, Alicja ; Harholt, Jesper ; Amelot, Nicolas ; Immerzeel, Peter ; Ryden, Peter ; Endo, Satoshi ; Ibatullin, Farid M. ; Brumer, Harry ; del Campillo, Elena ; Master, Emma R. ; Scheller, Henrik Vibe ; Sundberg, Bjorn ; Teeri, Tuula T. ; Mellerowicz, Ewa J. / KORRIGAN1 and its Aspen Homolog PttCel9A1 Decrease Cellulose Crystallinity in Arabidopsis Stems. In: PLANT AND CELL PHYSIOLOGY. 2009 ; Vol. 50, No. 6. pp. 1099-1115.

@article{b413c2395ab64f74b5a8b1100344994f,

title = "KORRIGAN1 and its Aspen Homolog PttCel9A1 Decrease Cellulose Crystallinity in Arabidopsis Stems",

abstract = "KORRIGAN1 (KOR1) is a membrane-bound cellulase implicated in cellulose biosynthesis. PttCel9A1 from hybrid aspen (Populus tremula L. tremuloides Michx.) has high sequence similarity to KOR1 and we demonstrate here that it complements kor1-1 mutants, indicating that it is a KOR1 ortholog. We investigated the function of PttCel9A1/KOR1 in Arabidopsis secondary growth using transgenic lines expressing 35S::PttCel9A1 and the KOR1 mutant line irx2-2. The presence of elevated levels of PttCel9A1/KOR1 in secondary walls of 35S::PttCel9A1 lines was confirmed by in muro visualization of cellulase activity. Compared with the wild type, 35S::PttCel9A1 lines had higher trifluoroacetic acid (TFA)-hydrolyzable glucan contents, similar Updegraff cellulose contents and lower cellulose crystallinity indices, as determined by (13)C solid-state nuclear magnetic resonance (NMR) spectroscopy. irx2-2 mutants had wild-type TFA-hydrolyzable glucan contents, but reduced Updegraff cellulose contents and higher than wild-type cellulose crystallinity indices. The data support the hypothesis that PttCel9A1/KOR1 activity is present in cell walls, where it facilitates cellulose biosynthesis in a way that increases the amount of non-crystalline cellulose.",

keywords = "Aspen, Cellulase, Cellulose, Cell wall, Populus, Wood formation, CARBOHYDRATE-ACTIVE ENZYMES, SECONDARY CELL-WALL, HIGHER-PLANTS, C-13 NMR, WOOD FORMATION, BIOSYNTHESIS, EXPRESSION, THALIANA, ENDO-1,4-BETA-GLUCANASE, GENES",

author = "Junko Takahashi and Rudsander, {Ulla J.} and Mattias Hedenstrom and Alicja Banasiak and Jesper Harholt and Nicolas Amelot and Peter Immerzeel and Peter Ryden and Satoshi Endo and Ibatullin, {Farid M.} and Harry Brumer and {del Campillo}, Elena and Master, {Emma R.} and Scheller, {Henrik Vibe} and Bjorn Sundberg and Teeri, {Tuula T.} and Mellerowicz, {Ewa J.}",

year = "2009",

month = jun,

doi = "10.1093/pcp/pcp062",

language = "English",

volume = "50",

pages = "1099--1115",

journal = "PLANT AND CELL PHYSIOLOGY",

issn = "0032-0781",

number = "6",

}

Takahashi, J, Rudsander, UJ, Hedenstrom, M, Banasiak, A, Harholt, J, Amelot, N, Immerzeel, P, Ryden, P, Endo, S, Ibatullin, FM, Brumer, H, del Campillo, E, Master, ER, Scheller, HV, Sundberg, B, Teeri, TT & Mellerowicz, EJ 2009, 'KORRIGAN1 and its Aspen Homolog PttCel9A1 Decrease Cellulose Crystallinity in Arabidopsis Stems', PLANT AND CELL PHYSIOLOGY, vol. 50, no. 6, pp. 1099-1115. https://doi.org/10.1093/pcp/pcp062

KORRIGAN1 and its Aspen Homolog PttCel9A1 Decrease Cellulose Crystallinity in Arabidopsis Stems. / Takahashi, Junko; Rudsander, Ulla J.; Hedenstrom, Mattias; Banasiak, Alicja; Harholt, Jesper; Amelot, Nicolas; Immerzeel, Peter; Ryden, Peter; Endo, Satoshi; Ibatullin, Farid M.; Brumer, Harry; del Campillo, Elena; Master, Emma R.; Scheller, Henrik Vibe; Sundberg, Bjorn; Teeri, Tuula T.; Mellerowicz, Ewa J.

In: PLANT AND CELL PHYSIOLOGY, Vol. 50, No. 6, 06.2009, p. 1099-1115.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - KORRIGAN1 and its Aspen Homolog PttCel9A1 Decrease Cellulose Crystallinity in Arabidopsis Stems

AU - Takahashi, Junko

AU - Rudsander, Ulla J.

AU - Hedenstrom, Mattias

AU - Banasiak, Alicja

AU - Harholt, Jesper

AU - Amelot, Nicolas

AU - Immerzeel, Peter

AU - Ryden, Peter

AU - Endo, Satoshi

AU - Ibatullin, Farid M.

AU - Brumer, Harry

AU - del Campillo, Elena

AU - Master, Emma R.

AU - Scheller, Henrik Vibe

AU - Sundberg, Bjorn

AU - Teeri, Tuula T.

AU - Mellerowicz, Ewa J.

PY - 2009/6

Y1 - 2009/6

N2 - KORRIGAN1 (KOR1) is a membrane-bound cellulase implicated in cellulose biosynthesis. PttCel9A1 from hybrid aspen (Populus tremula L. tremuloides Michx.) has high sequence similarity to KOR1 and we demonstrate here that it complements kor1-1 mutants, indicating that it is a KOR1 ortholog. We investigated the function of PttCel9A1/KOR1 in Arabidopsis secondary growth using transgenic lines expressing 35S::PttCel9A1 and the KOR1 mutant line irx2-2. The presence of elevated levels of PttCel9A1/KOR1 in secondary walls of 35S::PttCel9A1 lines was confirmed by in muro visualization of cellulase activity. Compared with the wild type, 35S::PttCel9A1 lines had higher trifluoroacetic acid (TFA)-hydrolyzable glucan contents, similar Updegraff cellulose contents and lower cellulose crystallinity indices, as determined by (13)C solid-state nuclear magnetic resonance (NMR) spectroscopy. irx2-2 mutants had wild-type TFA-hydrolyzable glucan contents, but reduced Updegraff cellulose contents and higher than wild-type cellulose crystallinity indices. The data support the hypothesis that PttCel9A1/KOR1 activity is present in cell walls, where it facilitates cellulose biosynthesis in a way that increases the amount of non-crystalline cellulose.

AB - KORRIGAN1 (KOR1) is a membrane-bound cellulase implicated in cellulose biosynthesis. PttCel9A1 from hybrid aspen (Populus tremula L. tremuloides Michx.) has high sequence similarity to KOR1 and we demonstrate here that it complements kor1-1 mutants, indicating that it is a KOR1 ortholog. We investigated the function of PttCel9A1/KOR1 in Arabidopsis secondary growth using transgenic lines expressing 35S::PttCel9A1 and the KOR1 mutant line irx2-2. The presence of elevated levels of PttCel9A1/KOR1 in secondary walls of 35S::PttCel9A1 lines was confirmed by in muro visualization of cellulase activity. Compared with the wild type, 35S::PttCel9A1 lines had higher trifluoroacetic acid (TFA)-hydrolyzable glucan contents, similar Updegraff cellulose contents and lower cellulose crystallinity indices, as determined by (13)C solid-state nuclear magnetic resonance (NMR) spectroscopy. irx2-2 mutants had wild-type TFA-hydrolyzable glucan contents, but reduced Updegraff cellulose contents and higher than wild-type cellulose crystallinity indices. The data support the hypothesis that PttCel9A1/KOR1 activity is present in cell walls, where it facilitates cellulose biosynthesis in a way that increases the amount of non-crystalline cellulose.

KW - Aspen

KW - Cellulase

KW - Cellulose

KW - Cell wall

KW - Populus

KW - Wood formation

KW - CARBOHYDRATE-ACTIVE ENZYMES

KW - SECONDARY CELL-WALL

KW - HIGHER-PLANTS

KW - C-13 NMR

KW - WOOD FORMATION

KW - BIOSYNTHESIS

KW - EXPRESSION

KW - THALIANA

KW - ENDO-1,4-BETA-GLUCANASE

KW - GENES

U2 - 10.1093/pcp/pcp062

DO - 10.1093/pcp/pcp062

M3 - Article

VL - 50

SP - 1099

EP - 1115

JO - PLANT AND CELL PHYSIOLOGY

JF - PLANT AND CELL PHYSIOLOGY

SN - 0032-0781

IS - 6

ER -

KORRIGAN1 and its Aspen Homolog PttCel9A1 Decrease Cellulose Crystallinity in Arabidopsis Stems

Abstract

Keywords

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