KORRIGAN1 and its Aspen Homolog PttCel9A1 Decrease Cellulose Crystallinity in Arabidopsis Stems

Junko Takahashi, Ulla J. Rudsander, Mattias Hedenstrom, Alicja Banasiak, Jesper Harholt, Nicolas Amelot, Peter Immerzeel, Peter Ryden, Satoshi Endo, Farid M. Ibatullin, Harry Brumer, Elena del Campillo, Emma R. Master, Henrik Vibe Scheller, Bjorn Sundberg, Tuula T. Teeri, Ewa J. Mellerowicz*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

KORRIGAN1 (KOR1) is a membrane-bound cellulase implicated in cellulose biosynthesis. PttCel9A1 from hybrid aspen (Populus tremula L. tremuloides Michx.) has high sequence similarity to KOR1 and we demonstrate here that it complements kor1-1 mutants, indicating that it is a KOR1 ortholog. We investigated the function of PttCel9A1/KOR1 in Arabidopsis secondary growth using transgenic lines expressing 35S::PttCel9A1 and the KOR1 mutant line irx2-2. The presence of elevated levels of PttCel9A1/KOR1 in secondary walls of 35S::PttCel9A1 lines was confirmed by in muro visualization of cellulase activity. Compared with the wild type, 35S::PttCel9A1 lines had higher trifluoroacetic acid (TFA)-hydrolyzable glucan contents, similar Updegraff cellulose contents and lower cellulose crystallinity indices, as determined by (13)C solid-state nuclear magnetic resonance (NMR) spectroscopy. irx2-2 mutants had wild-type TFA-hydrolyzable glucan contents, but reduced Updegraff cellulose contents and higher than wild-type cellulose crystallinity indices. The data support the hypothesis that PttCel9A1/KOR1 activity is present in cell walls, where it facilitates cellulose biosynthesis in a way that increases the amount of non-crystalline cellulose.

Original languageEnglish
Pages (from-to)1099-1115
Number of pages17
JournalPLANT AND CELL PHYSIOLOGY
Volume50
Issue number6
DOIs
Publication statusPublished - Jun 2009
MoE publication typeA1 Journal article-refereed

Keywords

  • Aspen
  • Cellulase
  • Cellulose
  • Cell wall
  • Populus
  • Wood formation
  • CARBOHYDRATE-ACTIVE ENZYMES
  • SECONDARY CELL-WALL
  • HIGHER-PLANTS
  • C-13 NMR
  • WOOD FORMATION
  • BIOSYNTHESIS
  • EXPRESSION
  • THALIANA
  • ENDO-1,4-BETA-GLUCANASE
  • GENES

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