Kinetic modeling and microbiological study of two-step nitrification in a membrane bioreactor and hybrid moving bed biofilm reactor-membrane bioreactor for wastewater treatment

Research output: Contribution to journalArticleScientificpeer-review


  • J. C. Leyva-Díaz
  • A. González-Martínez
  • J. González-López
  • M. M. Muñío
  • J. M. Poyatos

Research units

  • University of Granada


A membrane bioreactor (MBR), a hybrid moving bed biofilm reactor-membrane bioreactor (MBBR-MBR) containing carriers in the anoxic and aerobic zones of the bioreactor and a hybrid MBBR-MBR which contained carriers only in the aerobic zone were used in parallel with the same urban wastewater and compared. The reactors operated with a hydraulic retention time (HRT) of 9.5h. Kinetic parameters for heterotrophic and autotrophic biomasses, mainly ammonium-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB), were evaluated and related to organic matter and nutrients removals. The microbial communities of each wastewater treatment plant (WWTP) were analyzed by 454 pyrosequencing methods to detect and quantify the contribution of nitrifying bacteria in the total bacterial community. All three systems showed similar performance in terms of pollutant removal although the hybrid MBBR-MBR containing carriers only in the aerobic zone of the bioreactor (WWTP 3) showed the best performance from the point of view of the kinetics of heterotrophic and nitrite-oxidizing bacteria, with values of μm,H=0.02665h-1, KM=8.88081mgO2L-1, μm,NOB=0.53690h-1 and KNOB=2.16702mgNL-1. It supported the efficiencies of chemical oxygen demand (COD) and total nitrogen (TN) removals and the concentrations of nitrite and nitrate in the different effluents.


Original languageEnglish
Pages (from-to)692-702
Number of pages11
JournalChemical Engineering Journal
Publication statusPublished - 1 Jan 2015
MoE publication typeA1 Journal article-refereed

    Research areas

  • Kinetic parameters, Membrane bioreactor, Moving bed biofilm reactor, Nitrogen removal, Two-step nitrification, Wastewater treatment

ID: 8885648