KatF (σs) synthesis in Escherichia coli is subject to posttranscriptional regulation

Peter C. Loewen; Ingemar von Ossowski; Jacek Switala; Michael R. Mulvey

doi:10.1128/JB.175.7.2150-2153.1993

KatF (σ^s) synthesis in Escherichia coli is subject to posttranscriptional regulation

Peter C. Loewen^*, Ingemar von Ossowski, Jacek Switala, Michael R. Mulvey

^*Corresponding author for this work

Not published at Aalto University

University of Manitoba

Research output: Contribution to journal › Comment/debate › Scientific › peer-review

Abstract

A transcriptional fusion of katF to the lacZ gene was expressed at increasingly higher levels throughout the exponential phase, but a translational fusion was expressed at low levels during exponential-phase growth and was induced 160-fold during the transition to stationary phase, implicating a posttranscriptional mechanism in the regulation of KatF synthesis. Mutational analyses suggested that the initiation codon of katF is the second ATG in the previously identified open reading frame.

Original language	English
Pages (from-to)	2150-2153
Number of pages	4
Journal	Journal of Bacteriology
Volume	175
Issue number	7
DOIs	https://doi.org/10.1128/JB.175.7.2150-2153.1993
Publication status	Published - Apr 1993
MoE publication type	B1 Non-refereed journal articles

Keywords

MESSENGER-RNA
TRANSCRIPTION
EXPRESSION
INITIATION
PROTEIN
SINGLE

Access to Document

10.1128/JB.175.7.2150-2153.1993

Cite this

@article{1e3ba97a2dfb454a882a735b4095a942,

title = "KatF (σs) synthesis in Escherichia coli is subject to posttranscriptional regulation",

abstract = "A transcriptional fusion of katF to the lacZ gene was expressed at increasingly higher levels throughout the exponential phase, but a translational fusion was expressed at low levels during exponential-phase growth and was induced 160-fold during the transition to stationary phase, implicating a posttranscriptional mechanism in the regulation of KatF synthesis. Mutational analyses suggested that the initiation codon of katF is the second ATG in the previously identified open reading frame.",

keywords = "MESSENGER-RNA, TRANSCRIPTION, EXPRESSION, INITIATION, PROTEIN, SINGLE",

author = "Loewen, {Peter C.} and {von Ossowski}, Ingemar and Jacek Switala and Mulvey, {Michael R.}",

year = "1993",

month = apr,

doi = "10.1128/JB.175.7.2150-2153.1993",

language = "English",

volume = "175",

pages = "2150--2153",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "7",

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TY - JOUR

T1 - KatF (σs) synthesis in Escherichia coli is subject to posttranscriptional regulation

AU - Loewen, Peter C.

AU - von Ossowski, Ingemar

AU - Switala, Jacek

AU - Mulvey, Michael R.

PY - 1993/4

Y1 - 1993/4

N2 - A transcriptional fusion of katF to the lacZ gene was expressed at increasingly higher levels throughout the exponential phase, but a translational fusion was expressed at low levels during exponential-phase growth and was induced 160-fold during the transition to stationary phase, implicating a posttranscriptional mechanism in the regulation of KatF synthesis. Mutational analyses suggested that the initiation codon of katF is the second ATG in the previously identified open reading frame.

AB - A transcriptional fusion of katF to the lacZ gene was expressed at increasingly higher levels throughout the exponential phase, but a translational fusion was expressed at low levels during exponential-phase growth and was induced 160-fold during the transition to stationary phase, implicating a posttranscriptional mechanism in the regulation of KatF synthesis. Mutational analyses suggested that the initiation codon of katF is the second ATG in the previously identified open reading frame.

KW - MESSENGER-RNA

KW - TRANSCRIPTION

KW - EXPRESSION

KW - INITIATION

KW - PROTEIN

KW - SINGLE

U2 - 10.1128/JB.175.7.2150-2153.1993

DO - 10.1128/JB.175.7.2150-2153.1993

M3 - Comment/debate

SN - 0021-9193

VL - 175

SP - 2150

EP - 2153

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 7

ER -