Abstract
Genes that are highly expressed on glucose-containing media were isolated from the filamentous fungus, Trichoderma reesei. A cDNA bank was prepared from glucose-grown fungus, the bank was screened with the same cDNA as a probe, and clones giving the strongest signal were isolated. This resulted in the isolation of previously uncharacterized genes. Five of the genes, representing the most abundant transcripts, corresponded to 1-3% of the total mRNA population and were clearly more highly expressed than the phosphoglycerate kinase-encoding gene (pgk1) of T. reesei. Based on sequence homology, one of the genes was identified as tef1, encoding translation elongation factor 1 alpha (TEF). The T. reesei TEF is most related to the Mucor racemosus TEF3, showing an overall amino acid similarity of 85%. Interestingly, an exon of only 2 bp seems to be present in T. reesei tef1, comprising the first 2 bp of the Gly(15) codon.
| Original language | English |
|---|---|
| Pages (from-to) | 313-318 |
| Number of pages | 6 |
| Journal | Gene |
| Volume | 136 |
| Issue number | 1-2 |
| Publication status | Published - 22 Dec 1993 |
| MoE publication type | B1 Non-refereed journal articles |
Keywords
- FILAMENTOUS FUNGI
- CLONING METHOD
- ABUNDANT MESSENGER-RNA
- GENE EXPRESSION
- EXON SIZE
- MOLECULAR-CLONING
- SEQUENCE
- ENDOGLUCANASE
- SYSTEM
- KINASE