ISOLATION OF TRICHODERMA-REESEI GENES HIGHLY EXPRESSED ON GLUCOSE-CONTAINING MEDIA - CHARACTERIZATION OF THE TEF1 GENE ENCODING TRANSLATION ELONGATION-FACTOR 1-ALPHA

T NAKARI*, Edward Alatalo, ME PENTTILA

*Corresponding author for this work

Research output: Contribution to journalComment/debateScientificpeer-review

Abstract

Genes that are highly expressed on glucose-containing media were isolated from the filamentous fungus, Trichoderma reesei. A cDNA bank was prepared from glucose-grown fungus, the bank was screened with the same cDNA as a probe, and clones giving the strongest signal were isolated. This resulted in the isolation of previously uncharacterized genes. Five of the genes, representing the most abundant transcripts, corresponded to 1-3% of the total mRNA population and were clearly more highly expressed than the phosphoglycerate kinase-encoding gene (pgk1) of T. reesei. Based on sequence homology, one of the genes was identified as tef1, encoding translation elongation factor 1 alpha (TEF). The T. reesei TEF is most related to the Mucor racemosus TEF3, showing an overall amino acid similarity of 85%. Interestingly, an exon of only 2 bp seems to be present in T. reesei tef1, comprising the first 2 bp of the Gly(15) codon.

Original languageEnglish
Pages (from-to)313-318
Number of pages6
JournalGene
Volume136
Issue number1-2
Publication statusPublished - 22 Dec 1993
MoE publication typeA1 Journal article-refereed

Keywords

  • FILAMENTOUS FUNGI
  • CLONING METHOD
  • ABUNDANT MESSENGER-RNA
  • GENE EXPRESSION
  • EXON SIZE
  • MOLECULAR-CLONING
  • SEQUENCE
  • ENDOGLUCANASE
  • SYSTEM
  • KINASE

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