Ionic mechanisms in mouse rod photoreceptor signaling

Frans Vinberg

    Research output: ThesisDoctoral ThesisCollection of Articles

    Abstract

    Photoreceptor cells are an example of biological transducer devices: they transform photon energy into an electrical signal and transmit it to higher-order neurons. Vertebrate photoreceptor cells can be categorized into two classes, rods and cones. The rod photoreceptors are extremely sensitive to light, whereas cones are faster than rods and can function under bright ambient illumination. The rod photoreceptor cell is a convenient model for studying modulation of physiological transduction and transmission processes because 1) the rod's natural input signal, light, can be applied quantitatively and 2) the absorption of only one or a few photons by the visual pigment molecules in the cell's outer segment is transformed into a measurable change of the rod's membrane potential (Vm). The gain of the photon-to-Vm conversion in rods is rapidly (in a fraction of a second) modulated by several ionic feedback mechanisms. The mechanisms involved in rod signal generation and feedback signaling were investigated in the present work by recording rods' electrical responses to light stimuli from intact mouse retinas with transretinal electroretinogram (ex vivo ERG). Several negative feedback mechanisms that accelerate a rod's response recovery after light stimuli rely on the light-induced decline in the calcium ion (Ca2+) concentration in the rod outer segment. Further, some voltage- and Ca2+ -dependent mechanisms in the rod inner segment plasma membrane modulate the gain of the photon-to-Vm conversion. In this thesis the specificity of the known Ca2+ signaling mechanisms, and Ca2+ dependency of the reaction that rate-limits the rod's recovery after bright stimuli were investigated. It was found that the transition metal ion Co2+ can mediate the known Ca2+ dependent negative feedback mechanisms in the rod outer segment, and that a certain minimum amount of Ca2+ is necessary in setting the physiological value of the speed of the rate-limiting recovery reaction. The role of the inner segment ionic channels in generating the rod ERG response was also elucidated. It was shown that the hyperpolarization activated (h) channels in the rod inner segment participate in the generation of a fast transient component that is evident in the rod ERG response to bright flashes. Instead, voltage-dependent Ca2+ channels or Ca2+ -activated potassium and chloride currents did not contribute to that component. Additionally, modulation of the direct electrical transmission between rods and cones was studied. The present work suggests that the electrical connection between rods and cones can be closed by light in the mouse retina.
    Translated title of the contributionIonic mechanisms in mouse rod photoreceptor signaling
    Original languageEnglish
    QualificationDoctor's degree
    Awarding Institution
    • Aalto University
    Supervisors/Advisors
    • Koskelainen, Ari, Supervising Professor
    • Koskelainen, Ari, Thesis Advisor
    Publisher
    Print ISBNs978-952-60-4234-3
    Electronic ISBNs978-952-60-4235-0
    Publication statusPublished - 2011
    MoE publication typeG5 Doctoral dissertation (article)

    Keywords

    • photoreceptor
    • electrophysiology
    • electroretinogram
    • calcium signaling

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