Introduction of an electrochemical point-of-care assay for quantitative determination of paracetamol in finger-prick capillary whole blood samples

Johanna Kujala; Niklas Wester; Terhi J. Lohela; Mika Kurkela; Janne T. Backman; Björn Mikladal; Tomi Laurila; Jari Koskinen; Tuomas O. Lilius; Eija A. Kalso

doi:10.1111/bcp.15794

Introduction of an electrochemical point-of-care assay for quantitative determination of paracetamol in finger-prick capillary whole blood samples

Johanna Kujala, Niklas Wester, Terhi J. Lohela, Mika Kurkela, Janne T. Backman, Björn Mikladal, Tomi Laurila, Jari Koskinen, Tuomas O. Lilius^*, Eija A. Kalso

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

Aims: Measuring venous plasma paracetamol concentrations is time- and resource-consuming. We aimed to validate a novel electrochemical point-of-care (POC) assay for rapid paracetamol concentration determinations. Methods: Twelve healthy volunteers received 1 g oral paracetamol, and its concentrations were analysed 10 times over 12 h for capillary whole blood (POC), venous plasma (high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS)), and dried capillary blood (HPLC-MS/MS). Results: At concentrations >30 μM, POC showed upward biases of 20% (95% limits of agreement [LOA] −22 to 62) and 7% (95% LOA −23 to 38) compared with venous plasma and capillary blood HPLC-MS/MS, respectively. There were no significant differences between mean concentrations for the paracetamol elimination phase. Conclusions: Upward biases in POC compared with venous plasma HPLC-MS/MS were likely due to higher paracetamol concentrations in capillary blood than in venous plasma and to faulty individual sensors. The novel POC method is a promising tool for paracetamol concentration analysis.

Original language	English
Pages (from-to)	2933-2938
Number of pages	6
Journal	British Journal of Clinical Pharmacology
Volume	89
Issue number	9
Early online date	7 Jun 2023
DOIs	https://doi.org/10.1111/bcp.15794
Publication status	Published - Sept 2023
MoE publication type	A1 Journal article-refereed

Keywords

acetaminophen
capillary
diagnostics
intoxication
paracetamol
point-of-care
SWCNT

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Kujala, J., Wester, N., Lohela, T. J., Kurkela, M., Backman, J. T., Mikladal, B., Laurila, T., Koskinen, J., Lilius, T. O., & Kalso, E. A. (2023). Introduction of an electrochemical point-of-care assay for quantitative determination of paracetamol in finger-prick capillary whole blood samples. British Journal of Clinical Pharmacology, 89(9), 2933-2938. https://doi.org/10.1111/bcp.15794

@article{24d368fb59e547638113499649e76968,

title = "Introduction of an electrochemical point-of-care assay for quantitative determination of paracetamol in finger-prick capillary whole blood samples",

abstract = "Aims: Measuring venous plasma paracetamol concentrations is time- and resource-consuming. We aimed to validate a novel electrochemical point-of-care (POC) assay for rapid paracetamol concentration determinations. Methods: Twelve healthy volunteers received 1 g oral paracetamol, and its concentrations were analysed 10 times over 12 h for capillary whole blood (POC), venous plasma (high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS)), and dried capillary blood (HPLC-MS/MS). Results: At concentrations >30 μM, POC showed upward biases of 20% (95% limits of agreement [LOA] −22 to 62) and 7% (95% LOA −23 to 38) compared with venous plasma and capillary blood HPLC-MS/MS, respectively. There were no significant differences between mean concentrations for the paracetamol elimination phase. Conclusions: Upward biases in POC compared with venous plasma HPLC-MS/MS were likely due to higher paracetamol concentrations in capillary blood than in venous plasma and to faulty individual sensors. The novel POC method is a promising tool for paracetamol concentration analysis.",

keywords = "acetaminophen, capillary, diagnostics, intoxication, paracetamol, point-of-care, SWCNT",

author = "Johanna Kujala and Niklas Wester and Lohela, {Terhi J.} and Mika Kurkela and Backman, {Janne T.} and Bj{\"o}rn Mikladal and Tomi Laurila and Jari Koskinen and Lilius, {Tuomas O.} and Kalso, {Eija A.}",

note = "The authors would like to thank A/Prof. Mark Chatfield, University of Queensland, Australia, for his assistance with Stata code for Bland–Altman and Passing-Bablok analysis; and Laura Tervala, Department of Clinical Pharmacology, HUS Helsinki University Hospital, Helsinki, Finland; and Meeri Alanappa, Department of Clinical Pharmacology, HUS Helsinki University Hospital, Helsinki, Finland, for their skilful assistance.",

year = "2023",

month = sep,

doi = "10.1111/bcp.15794",

language = "English",

volume = "89",

pages = "2933--2938",

journal = "British Journal of Clinical Pharmacology",

issn = "0306-5251",

publisher = "Wiley-Blackwell",

number = "9",

}

Kujala, J, Wester, N, Lohela, TJ, Kurkela, M, Backman, JT, Mikladal, B, Laurila, T , Koskinen, J, Lilius, TO & Kalso, EA 2023, 'Introduction of an electrochemical point-of-care assay for quantitative determination of paracetamol in finger-prick capillary whole blood samples', British Journal of Clinical Pharmacology, vol. 89, no. 9, pp. 2933-2938. https://doi.org/10.1111/bcp.15794

Introduction of an electrochemical point-of-care assay for quantitative determination of paracetamol in finger-prick capillary whole blood samples. / Kujala, Johanna; Wester, Niklas; Lohela, Terhi J. et al.
In: British Journal of Clinical Pharmacology, Vol. 89, No. 9, 09.2023, p. 2933-2938.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - Introduction of an electrochemical point-of-care assay for quantitative determination of paracetamol in finger-prick capillary whole blood samples

AU - Kujala, Johanna

AU - Wester, Niklas

AU - Lohela, Terhi J.

AU - Kurkela, Mika

AU - Backman, Janne T.

AU - Mikladal, Björn

AU - Laurila, Tomi

AU - Koskinen, Jari

AU - Lilius, Tuomas O.

AU - Kalso, Eija A.

N1 - The authors would like to thank A/Prof. Mark Chatfield, University of Queensland, Australia, for his assistance with Stata code for Bland–Altman and Passing-Bablok analysis; and Laura Tervala, Department of Clinical Pharmacology, HUS Helsinki University Hospital, Helsinki, Finland; and Meeri Alanappa, Department of Clinical Pharmacology, HUS Helsinki University Hospital, Helsinki, Finland, for their skilful assistance.

PY - 2023/9

Y1 - 2023/9

N2 - Aims: Measuring venous plasma paracetamol concentrations is time- and resource-consuming. We aimed to validate a novel electrochemical point-of-care (POC) assay for rapid paracetamol concentration determinations. Methods: Twelve healthy volunteers received 1 g oral paracetamol, and its concentrations were analysed 10 times over 12 h for capillary whole blood (POC), venous plasma (high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS)), and dried capillary blood (HPLC-MS/MS). Results: At concentrations >30 μM, POC showed upward biases of 20% (95% limits of agreement [LOA] −22 to 62) and 7% (95% LOA −23 to 38) compared with venous plasma and capillary blood HPLC-MS/MS, respectively. There were no significant differences between mean concentrations for the paracetamol elimination phase. Conclusions: Upward biases in POC compared with venous plasma HPLC-MS/MS were likely due to higher paracetamol concentrations in capillary blood than in venous plasma and to faulty individual sensors. The novel POC method is a promising tool for paracetamol concentration analysis.

AB - Aims: Measuring venous plasma paracetamol concentrations is time- and resource-consuming. We aimed to validate a novel electrochemical point-of-care (POC) assay for rapid paracetamol concentration determinations. Methods: Twelve healthy volunteers received 1 g oral paracetamol, and its concentrations were analysed 10 times over 12 h for capillary whole blood (POC), venous plasma (high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS)), and dried capillary blood (HPLC-MS/MS). Results: At concentrations >30 μM, POC showed upward biases of 20% (95% limits of agreement [LOA] −22 to 62) and 7% (95% LOA −23 to 38) compared with venous plasma and capillary blood HPLC-MS/MS, respectively. There were no significant differences between mean concentrations for the paracetamol elimination phase. Conclusions: Upward biases in POC compared with venous plasma HPLC-MS/MS were likely due to higher paracetamol concentrations in capillary blood than in venous plasma and to faulty individual sensors. The novel POC method is a promising tool for paracetamol concentration analysis.

KW - acetaminophen

KW - capillary

KW - diagnostics

KW - intoxication

KW - paracetamol

KW - point-of-care

KW - SWCNT

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U2 - 10.1111/bcp.15794

DO - 10.1111/bcp.15794

M3 - Article

C2 - 37218304

AN - SCOPUS:85161489597

SN - 0306-5251

VL - 89

SP - 2933

EP - 2938

JO - British Journal of Clinical Pharmacology

JF - British Journal of Clinical Pharmacology

IS - 9

ER -

Introduction of an electrochemical point-of-care assay for quantitative determination of paracetamol in finger-prick capillary whole blood samples

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Introduction of an electrochemical point-of-care assay for quantitative determination of paracetamol in finger-prick capillary whole blood samples

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Aalto University Reports Findings in Hematology (Introduction of an electrochemical point-of-care assay for quantitative determination of paracetamol in finger-prick capillary whole blood samples)

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