Intrinsically-disordered N-termini in human parechovirus 1 capsid proteins bind encapsidated RNA

Research output: Contribution to journalArticleScientificpeer-review

Researchers

  • Shabih Shakeel
  • James D. Evans
  • Mark Hazelbaker
  • C. Cheng Kao
  • Robert C. Vaughan
  • Sarah J. Butcher

Research units

  • University of Helsinki
  • Medical Research Council
  • Indiana University Bloomington

Abstract

Human parechoviruses (HPeV) are picornaviruses with a highly-ordered RNA genome contained within icosahedrally-symmetric capsids. Ordered RNA structures have recently been shown to interact with capsid proteins VP1 and VP3 and facilitate virus assembly in HPeV1. Using an assay that combines reversible cross-linking, RNA affinity purification and peptide mass fingerprinting (RCAP), we mapped the RNA-interacting regions of the capsid proteins from the whole HPeV1 virion in solution. The intrinsically-disordered N-termini of capsid proteins VP1 and VP3, and unexpectedly, VP0, were identified to interact with RNA. Comparing these results to those obtained using recombinantly-expressed VP0 and VP1 confirmed the virion binding regions, and revealed unique RNA binding regions in the isolated VP0 not previously observed in the crystal structure of HPeV1. We used RNA fluorescence anisotropy to confirm the RNA-binding competency of each of the capsid proteins' N-termini. These findings suggests that dynamic interactions between the viral RNA and the capsid proteins modulate virus assembly, and suggest a novel role for VP0.

Details

Original languageEnglish
Article number5820
JournalScientific Reports
Volume8
Issue number1
Publication statusPublished - 1 Dec 2018
MoE publication typeA1 Journal article-refereed

ID: 32125692