Interactions between type A carbohydrate binding modules and cellulose studied with a quartz crystal microbalance with dissipation monitoring

Yu Zhang, Xiaoyan Wang, Peipei Wang, Junlong Song*, Yongcan Jin, Orlando J. Rojas

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)

Abstract

Abstract: The specific interaction between carbohydrate binding modules (CBMs) and substrates is of utmost importance due to it affects the biological activity of the parent enzymes and determines the chemo-mechanical properties of protein-cellulose composites. In this investigation, a quartz crystal microbalance with dissipation monitoring was employed to study, in situ and in real-time, the adsorption behaviors, conformational changes and associated thermodynamics that define the specific interactions between type A CBMs (CBM1 and CBM3) and cellulose (crystalline, CNC and nanofibrillated, CNF). CBM1 and CBM3 specifically bind to CNC and CNF substrates, with the CBM3 forming more rigid adsorbed layers at 25 °C. Despite the wide variation in adsorption enthalpy (ΔH) and entropy (ΔS), depending on the experimental conditions, a negative Gibbs free energy (ΔG) was determined from the adsorption isotherms for both CBMs. Particularly, the ΔH and ΔS associated with CBM3 adsorbing on CNF exhibited significant greater values than others due to cellulose chain disorder when swelling. The results further our understandings on the interactions between type A CBMs and cellulose substrates. Graphic abstract: [Figure not available: see fulltext.].

Original languageEnglish
Pages (from-to)3661-3675
Number of pages15
JournalCellulose
Volume27
Issue number7
Early online date26 Feb 2020
DOIs
Publication statusPublished - 1 May 2020
MoE publication typeA1 Journal article-refereed

Keywords

  • Carbohydrate binding module
  • Interaction
  • Nanocellulose
  • Protein-cellulose composites
  • Quartz crystal microbalance
  • Thermodynamics

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