Inkjet-fabricated coated reaction platform for colorimetric glucose detection

Microfluidic paper-based analytical devices (μPADs) provide a simple and low-cost platform for colorimetric glucose assays based on an enzymatic reaction. Most assays utilise filter paper-based reaction platforms, though coated platforms may enable fabrication of even smaller devices and thus lower liquid volumes and increased spatial resolution. In this study, we present a coated microfluidic reaction platform designed for colorimetric detection of glucose, which has been fabricated utilising speciality coated substrates and inkjet printing. The preliminary results show that glucose oxidation takes place on the coated substrates. The glucose assay, nonetheless, can be used to verify the performance of a coated reaction platform, after which the platform could be adapted for detection of other analytes. Evidence is collected to aid future optimisation of the coating formulation, pattern design, reagent application method, preservation of enzyme activity, colorimetric detection improvement to overcome high light scattering, which reduces colour intensity, and defining the detectable glucose range.