Inhibitory effect of lignin during cellulose bioconversion: The effect of lignin chemistry on non-productive enzyme adsorption

Jenni L. Rahikainen*, Raquel Martin-Sampedro, Harri Heikkinen, Stella Rovio, Kaisa Marjamaa, Tarja Tamminen, Orlando J. Rojas, Kristiina Kruus

*Corresponding author for this work

    Research output: Contribution to journalArticleScientificpeer-review

    280 Citations (Scopus)

    Abstract

    The effect of lignin as an inhibitory biopolymer for the enzymatic hydrolysis of lignocellulosic biomass was studied; specially addressing the role of lignin in non-productive enzyme adsorption. Botanical origin and biomass pre-treatment give rise to differences in lignin structure and the effect of these differences on enzyme binding and inhibition were elucidated. Lignin was isolated from steam explosion (SE) pre-treated and non-treated spruce and wheat straw and used for the preparation of ultrathin films for enzyme binding studies. Binding of Trichoderma reesei Cel7A (CBHI) and the corresponding Cel7A-core, lacking the linker and the cellulose-binding domain, to the lignin films was monitored using a quartz crystal microbalance (QCM). SE pre-treatment altered the lignin structure, leading to increased enzyme adsorption. Thus, the positive effect of SE pre-treatment, opening the cell wall matrix to make polysaccharides more accessible, may be compromised by the structural changes of lignin that increase non-productive enzyme adsorption. (C) 2013 Elsevier Ltd. All rights reserved.

    Original languageEnglish
    Pages (from-to)270-278
    Number of pages9
    JournalBioresource Technology
    Volume133
    DOIs
    Publication statusPublished - Apr 2013
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Lignin
    • Steam explosion pre-treatment
    • Cellulase binding
    • Trichoderma reesei Cel7A
    • Carbohydrate binding module
    • STEAM-EXPLOSION PRETREATMENT
    • TRICHODERMA-REESEI
    • CELLOBIOHYDROLASE-I
    • WHEAT-STRAW
    • THIN-FILMS
    • HYDROLYSIS
    • WOOD
    • SOFTWOOD
    • SURFACE
    • DELIGNIFICATION

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