Inhibitory effect of lignin during cellulose bioconversion: The effect of lignin chemistry on non-productive enzyme adsorption

Research output: Contribution to journalArticleScientificpeer-review


Research units

  • North Carolina State University
  • VTT Technical Research Centre of Finland


The effect of lignin as an inhibitory biopolymer for the enzymatic hydrolysis of lignocellulosic biomass was studied; specially addressing the role of lignin in non-productive enzyme adsorption. Botanical origin and biomass pre-treatment give rise to differences in lignin structure and the effect of these differences on enzyme binding and inhibition were elucidated. Lignin was isolated from steam explosion (SE) pre-treated and non-treated spruce and wheat straw and used for the preparation of ultrathin films for enzyme binding studies. Binding of Trichoderma reesei Cel7A (CBHI) and the corresponding Cel7A-core, lacking the linker and the cellulose-binding domain, to the lignin films was monitored using a quartz crystal microbalance (QCM). SE pre-treatment altered the lignin structure, leading to increased enzyme adsorption. Thus, the positive effect of SE pre-treatment, opening the cell wall matrix to make polysaccharides more accessible, may be compromised by the structural changes of lignin that increase non-productive enzyme adsorption. (C) 2013 Elsevier Ltd. All rights reserved.


Original languageEnglish
Pages (from-to)270-278
Number of pages9
JournalBioresource Technology
Publication statusPublished - Apr 2013
MoE publication typeA1 Journal article-refereed

    Research areas

  • Lignin, Steam explosion pre-treatment, Cellulase binding, Trichoderma reesei Cel7A, Carbohydrate binding module, STEAM-EXPLOSION PRETREATMENT, TRICHODERMA-REESEI, CELLOBIOHYDROLASE-I, WHEAT-STRAW, THIN-FILMS, HYDROLYSIS, WOOD, SOFTWOOD, SURFACE, DELIGNIFICATION

ID: 10400778