Inhibition of human natural killer cell activity by cereulide, an emetic toxin from Bacillus cereus

A Paananen*, R Mikkola, T Sareneva, S Matikainen, M Hess, M Andersson, Petro Julkunen, MS Salkinoja-Salonen, T Timonen

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

The lipophilic toxin, cereulide, emitted by emetic food poisoning causing strains of Bacillus cereus , is a powerful mitochondria toxin. It is highly lipophilic and rapidly absorbed from the gut into the bloodstream. We tested how this toxin influences natural killer (NK) cells, which are important effectors in defence against infections and malignancy. Cereulide inhibited cytotoxicity and cytokine production of natural killer cells, caused swelling of natural killer cell mitochondria, and eventually induced natural killer cell apoptosis. The suppressive effect on cytotoxicity was fast and toxic concentration low, 20-30 mug/l. As the emesis causing concentration of cereulide is around 10 mug/kg of total body mass, our results suggest that emesis causing or even lower doses of cereulide may also have a systemic natural killer cell suppressive effect.

Original languageEnglish
Pages (from-to)420-428
Number of pages9
JournalCLINICAL AND EXPERIMENTAL IMMUNOLOGY
Volume129
Issue number3
Publication statusPublished - Sep 2002
MoE publication typeA1 Journal article-refereed

Keywords

  • cereulide
  • NK cell
  • IL-15
  • IL-18
  • HEP-2 VACUOLATION FACTOR
  • STREPTOMYCES-GRISEUS
  • INDOOR AIR
  • DODECADEPSIPEPTIDE
  • MITOCHONDRIA
  • VALINOMYCIN
  • CLONING

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