Indocyanine Green-Loaded Liposomes for Light-Triggered Drug Release

Tatu Lajunen*, Leena Stiina Kontturi, Lauri Viitala, Moutusi Manna, Oana Cramariuc, Tomasz Róg, Alex Bunker, Timo Laaksonen, Tapani Viitala, Lasse Murtomäki, Arto Urtti

*Corresponding author for this work

    Research output: Contribution to journalArticleScientificpeer-review

    59 Citations (Scopus)

    Abstract

    Light-triggered drug delivery systems enable site-specific and time-controlled drug release. In previous work, we have achieved this with liposomes containing gold nanoparticles in the aqueous core. Gold nanoparticles absorb near-infrared light and release the energy as heat that increases the permeability of the liposomal bilayer, thus releasing the contents of the liposome. In this work, we replaced the gold nanoparticles with the clinically approved imaging agent indocyanine green (ICG). The ICG liposomes were stable at storage conditions (4-22 °C) and at body temperature, and fast near-infrared (IR) light-triggered drug release was achieved with optimized phospholipid composition and a 1:50 ICG-to-lipid molar ratio. Encapsulated small molecular calcein and FITC-dextran (up to 20 kDa) were completely released from the liposomes after light exposure for 15 s. Location of ICG in the PEG layer of the liposomes was simulated with molecular dynamics. ICG has important benefits as a light-triggering agent in liposomes: fast content release, improved stability, improved possibility of liposomal size control, regulatory approval to use in humans, and the possibility of imaging the in vivo location of the liposomes based on the fluorescence of ICG. Near-infrared light used as a triggering mechanism has good tissue penetration and safety. Thus, ICG liposomes are an attractive option for light-controlled and efficient delivery of small and large drug molecules.

    Original languageEnglish
    Pages (from-to)2095-2107
    Number of pages13
    JournalMOLECULAR PHARMACEUTICS
    Volume13
    Issue number6
    DOIs
    Publication statusPublished - 6 Jun 2016
    MoE publication typeA1 Journal article-refereed

    Keywords

    • indocyanine green
    • light activation
    • liposome
    • macromolecules
    • molecular dynamics
    • triggered release

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  • Cite this

    Lajunen, T., Kontturi, L. S., Viitala, L., Manna, M., Cramariuc, O., Róg, T., Bunker, A., Laaksonen, T., Viitala, T., Murtomäki, L., & Urtti, A. (2016). Indocyanine Green-Loaded Liposomes for Light-Triggered Drug Release. MOLECULAR PHARMACEUTICS, 13(6), 2095-2107. https://doi.org/10.1021/acs.molpharmaceut.6b00207