Indocyanine green-assisted and LED-light-activated antibacterial photodynamic therapy reduces dental plaque

Sakari Nikinmaa; Niina Moilanen; Timo Sorsa; Juha Rantala; Heikki Alapulli; Anja Kotiranta; Petri Auvinen; Esko Kankuri; Jukka H. Meurman; Tommi Pätilä

doi:10.3390/dj9050052

Indocyanine green-assisted and LED-light-activated antibacterial photodynamic therapy reduces dental plaque

Sakari Nikinmaa, Niina Moilanen, Timo Sorsa, Juha Rantala, Heikki Alapulli, Anja Kotiranta, Petri Auvinen, Esko Kankuri^*, Jukka H. Meurman, Tommi Pätilä

^*Corresponding author for this work

Department of Neuroscience and Biomedical Engineering

Research output: Contribution to journal › Article › Scientific › peer-review

13 Citations (Scopus)

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Abstract

Aim: This study aimed to determine the feasibility and first efficacy of indocyanine green (ICG)-assisted antimicrobial photodynamictherapy (aPDT) as activated using LED light to the dental plaque. Methods: Fifteen healthy adults were assigned to this four-day randomized study. After rinsing with ICG, 100 J/cm² of 810 nm LED light was applied to the aPDT-treatment area. Plaque area and gingival crevicular fluid (GCF) matrix metalloproteinase-8 (MMP-8) were measured, and plaque bacteriomes before and after the study were analyzed using 16S rRNA sequencing. Results: aPDT administration was preformed successfully and plaque-specifically with the combination of ICG and the applicator. Total plaque area and endpoint MMP-8 levels were reduced on the aPDT-treatment side. aPDT reduced Streptococcus, Acinetobacteria, Capnocytophaga, and Rothia bacteria species in plaques. Conclusion: ICG-assisted aPDT reduces plaque forming bacteria and exerts anti-inflammatory and anti-proteolytic effects.

Original language	English
Article number	52
Number of pages	11
Journal	Dentistry Journal
Volume	9
Issue number	5
DOIs	https://doi.org/10.3390/dj9050052
Publication status	Published - May 2021
MoE publication type	A1 Journal article-refereed

Keywords

Antibacterial photodynamic therapy
Dental plaque
Gingivitis

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10.3390/dj9050052

Indocyanine Green-Assisted.dentistry-09-00052-v2-2Final published version, 1.19 MBLicence: CC BY

Cite this

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title = "Indocyanine green-assisted and LED-light-activated antibacterial photodynamic therapy reduces dental plaque",

abstract = "Aim: This study aimed to determine the feasibility and first efficacy of indocyanine green (ICG)-assisted antimicrobial photodynamictherapy (aPDT) as activated using LED light to the dental plaque. Methods: Fifteen healthy adults were assigned to this four-day randomized study. After rinsing with ICG, 100 J/cm2 of 810 nm LED light was applied to the aPDT-treatment area. Plaque area and gingival crevicular fluid (GCF) matrix metalloproteinase-8 (MMP-8) were measured, and plaque bacteriomes before and after the study were analyzed using 16S rRNA sequencing. Results: aPDT administration was preformed successfully and plaque-specifically with the combination of ICG and the applicator. Total plaque area and endpoint MMP-8 levels were reduced on the aPDT-treatment side. aPDT reduced Streptococcus, Acinetobacteria, Capnocytophaga, and Rothia bacteria species in plaques. Conclusion: ICG-assisted aPDT reduces plaque forming bacteria and exerts anti-inflammatory and anti-proteolytic effects.",

keywords = "Antibacterial photodynamic therapy, Dental plaque, Gingivitis",

author = "Sakari Nikinmaa and Niina Moilanen and Timo Sorsa and Juha Rantala and Heikki Alapulli and Anja Kotiranta and Petri Auvinen and Esko Kankuri and Meurman, {Jukka H.} and Tommi P{\"a}til{\"a}",

note = "Funding Information: Funding: This research is part of a TUTL-project awarded to T.P. and funded by Aalto University (https://www.aalto.fi/en, accessed on 29 April 2021) and Tekes (https://www.businessfinland.fi/en, accessed on 29 April 2021; grant number 4364/31/2016). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = may,

doi = "10.3390/dj9050052",

language = "English",

volume = "9",

journal = "Dentistry Journal",

issn = "2304-6767",

publisher = "MDPI AG",

number = "5",

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TY - JOUR

T1 - Indocyanine green-assisted and LED-light-activated antibacterial photodynamic therapy reduces dental plaque

AU - Nikinmaa, Sakari

AU - Moilanen, Niina

AU - Sorsa, Timo

AU - Rantala, Juha

AU - Alapulli, Heikki

AU - Kotiranta, Anja

AU - Auvinen, Petri

AU - Kankuri, Esko

AU - Meurman, Jukka H.

AU - Pätilä, Tommi

N1 - Funding Information: Funding: This research is part of a TUTL-project awarded to T.P. and funded by Aalto University (https://www.aalto.fi/en, accessed on 29 April 2021) and Tekes (https://www.businessfinland.fi/en, accessed on 29 April 2021; grant number 4364/31/2016). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/5

Y1 - 2021/5

N2 - Aim: This study aimed to determine the feasibility and first efficacy of indocyanine green (ICG)-assisted antimicrobial photodynamictherapy (aPDT) as activated using LED light to the dental plaque. Methods: Fifteen healthy adults were assigned to this four-day randomized study. After rinsing with ICG, 100 J/cm2 of 810 nm LED light was applied to the aPDT-treatment area. Plaque area and gingival crevicular fluid (GCF) matrix metalloproteinase-8 (MMP-8) were measured, and plaque bacteriomes before and after the study were analyzed using 16S rRNA sequencing. Results: aPDT administration was preformed successfully and plaque-specifically with the combination of ICG and the applicator. Total plaque area and endpoint MMP-8 levels were reduced on the aPDT-treatment side. aPDT reduced Streptococcus, Acinetobacteria, Capnocytophaga, and Rothia bacteria species in plaques. Conclusion: ICG-assisted aPDT reduces plaque forming bacteria and exerts anti-inflammatory and anti-proteolytic effects.

AB - Aim: This study aimed to determine the feasibility and first efficacy of indocyanine green (ICG)-assisted antimicrobial photodynamictherapy (aPDT) as activated using LED light to the dental plaque. Methods: Fifteen healthy adults were assigned to this four-day randomized study. After rinsing with ICG, 100 J/cm2 of 810 nm LED light was applied to the aPDT-treatment area. Plaque area and gingival crevicular fluid (GCF) matrix metalloproteinase-8 (MMP-8) were measured, and plaque bacteriomes before and after the study were analyzed using 16S rRNA sequencing. Results: aPDT administration was preformed successfully and plaque-specifically with the combination of ICG and the applicator. Total plaque area and endpoint MMP-8 levels were reduced on the aPDT-treatment side. aPDT reduced Streptococcus, Acinetobacteria, Capnocytophaga, and Rothia bacteria species in plaques. Conclusion: ICG-assisted aPDT reduces plaque forming bacteria and exerts anti-inflammatory and anti-proteolytic effects.

KW - Antibacterial photodynamic therapy

KW - Dental plaque

KW - Gingivitis

UR - http://www.scopus.com/inward/record.url?scp=85108810170&partnerID=8YFLogxK

U2 - 10.3390/dj9050052

DO - 10.3390/dj9050052

M3 - Article

AN - SCOPUS:85108810170

SN - 2304-6767

VL - 9

JO - Dentistry Journal

JF - Dentistry Journal

IS - 5

M1 - 52

ER -

Indocyanine green-assisted and LED-light-activated antibacterial photodynamic therapy reduces dental plaque

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