In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei

Marleen Maras; André De Bruyn; Wouter Vervecken; Jaana Uusitalo; Merja Penttilä; Roger Busson; Piet Herdewijn; Roland Contreras

doi:10.1016/S0014-5793(99)00648-1

In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei

Marleen Maras, André De Bruyn, Wouter Vervecken, Jaana Uusitalo, Merja Penttilä, Roger Busson, Piet Herdewijn, Roland Contreras^*

^*Corresponding author for this work

Not published at Aalto University

Research output: Contribution to journal › Article › Scientific › peer-review

34 Citations (Scopus)

Abstract

The human N-acetylglucosaminyltransferase I gene was introduced in the genome of Trichoderma reesei strain VTT-D-80133, Expression was studied after induction from the cellobiohydrolase I promoter. Successful in vivo transfer of GlcNAc was demonstrated by analyzing the neutral N-glycans which were synthesized on cellobiohydrolase I. Final proof of the formation of GlcNAcMan₅GlcNAc₂ was obtained by NMR analysis. (C) 1999 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	365-370
Number of pages	6
Journal	FEBS Letters
Volume	452
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(99)00648-1
Publication status	Published - 11 Jun 1999
MoE publication type	A1 Journal article-refereed

Keywords

cellobiohydrolase I
fungus
N-acetylglucosaminyltransferase I
Trichoderma reesei
ASPERGILLUS-NIDULANS
CELLOBIOHYDROLASE-I
HIGH-RESOLUTION
OLIGOSACCHARIDES
GLYCOSYLATION
GLYCOPROTEINS
TRANSPORTER

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title = "In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei",

abstract = "The human N-acetylglucosaminyltransferase I gene was introduced in the genome of Trichoderma reesei strain VTT-D-80133, Expression was studied after induction from the cellobiohydrolase I promoter. Successful in vivo transfer of GlcNAc was demonstrated by analyzing the neutral N-glycans which were synthesized on cellobiohydrolase I. Final proof of the formation of GlcNAcMan5GlcNAc2 was obtained by NMR analysis. (C) 1999 Federation of European Biochemical Societies.",

keywords = "cellobiohydrolase I, fungus, N-acetylglucosaminyltransferase I, Trichoderma reesei, ASPERGILLUS-NIDULANS, CELLOBIOHYDROLASE-I, HIGH-RESOLUTION, OLIGOSACCHARIDES, GLYCOSYLATION, GLYCOPROTEINS, TRANSPORTER",

author = "Marleen Maras and {De Bruyn}, Andr{\'e} and Wouter Vervecken and Jaana Uusitalo and Merja Penttil{\"a} and Roger Busson and Piet Herdewijn and Roland Contreras",

year = "1999",

month = jun,

day = "11",

doi = "10.1016/S0014-5793(99)00648-1",

language = "English",

volume = "452",

pages = "365--370",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley & Sons",

number = "3",

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TY - JOUR

T1 - In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei

AU - Maras, Marleen

AU - De Bruyn, André

AU - Vervecken, Wouter

AU - Uusitalo, Jaana

AU - Penttilä, Merja

AU - Busson, Roger

AU - Herdewijn, Piet

AU - Contreras, Roland

PY - 1999/6/11

Y1 - 1999/6/11

N2 - The human N-acetylglucosaminyltransferase I gene was introduced in the genome of Trichoderma reesei strain VTT-D-80133, Expression was studied after induction from the cellobiohydrolase I promoter. Successful in vivo transfer of GlcNAc was demonstrated by analyzing the neutral N-glycans which were synthesized on cellobiohydrolase I. Final proof of the formation of GlcNAcMan5GlcNAc2 was obtained by NMR analysis. (C) 1999 Federation of European Biochemical Societies.

AB - The human N-acetylglucosaminyltransferase I gene was introduced in the genome of Trichoderma reesei strain VTT-D-80133, Expression was studied after induction from the cellobiohydrolase I promoter. Successful in vivo transfer of GlcNAc was demonstrated by analyzing the neutral N-glycans which were synthesized on cellobiohydrolase I. Final proof of the formation of GlcNAcMan5GlcNAc2 was obtained by NMR analysis. (C) 1999 Federation of European Biochemical Societies.

KW - cellobiohydrolase I

KW - fungus

KW - N-acetylglucosaminyltransferase I

KW - Trichoderma reesei

KW - ASPERGILLUS-NIDULANS

KW - CELLOBIOHYDROLASE-I

KW - HIGH-RESOLUTION

KW - OLIGOSACCHARIDES

KW - GLYCOSYLATION

KW - GLYCOPROTEINS

KW - TRANSPORTER

U2 - 10.1016/S0014-5793(99)00648-1

DO - 10.1016/S0014-5793(99)00648-1

M3 - Article

SN - 0014-5793

VL - 452

SP - 365

EP - 370

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei

Abstract

Keywords

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