In vitro toxicity of cereulide on porcine pancreatic Langerhans islets

Suvi M. Virtanen, Merja Roivainen, Maria A. Andersson, Petri Ylipaasto, Douwe Hoornstra, Raimo Mikkola, Mirja S. Salkinoja-Salonen*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Cereulide is a K+ ionophore cytotoxic and mitochondriotoxic to primary cells and cell lines of human and other mammalian origins. It is a heat-stable, highly lipophilic (log K-ow 5.96)peptide (1152gmol(-1)) produced by certain strains of Bacillus cereus, a bacterium connected to emetic food poisonings. In this study the pancreatic toxicity of purified cereulide, and cereulide-containing bacterial extracts, was studied using fetal porcine Langerhans islets in culture. Exposure to 1 ng ml(-1) of purified cereulide caused necrotic cell death of the islet cells impairing their insulin content within 2 days. Cell extracts of cereulide-positive B. cereus strains connected to food poisoning or isolated from foodstuffs were toxic, corresponding to their measured cereulide content. Extracts of B. cereus strains producing or not producing the B. cereus diarrheal toxin, but no cereulide, were tolerated by the porcine islet cultures up to concentrations 1000-fold higher compared to extracts from strains containing cereulide, and up to exposure times of 7 d. Cereulide thus was identified as the B. cereus-produced substance toxic towards porcine fetal Langerhans islets and beta cells. (c) 2008 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)1029-1037
Number of pages9
JournalTOXICON
Volume51
Issue number6
DOIs
Publication statusPublished - May 2008
MoE publication typeA1 Journal article-refereed

Keywords

  • cereulide
  • beta cell
  • Bacillus cereus
  • food poisoning
  • porcine pancreas
  • potassium carrier
  • ionophore
  • cytotoxicity
  • potato
  • BACILLUS-CEREUS
  • EMETIC TOXIN
  • BETA-CELLS
  • INSULIN-SECRETION
  • FOOD
  • POTASSIUM
  • DODECADEPSIPEPTIDE
  • DEPSIPEPTIDE
  • IONOPHORES
  • BIOASSAY

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