In vitro oxygen sensing using intraocular microrobots

Olgaç Ergeneman*, George Chatzipirpiridis, Juho Pokki, Marta Marin-Suárez, Georgios A. Sotiriou, Santiago Medina-Rodriguez, Jorge F.Fernández Sanchez, Alberto Fernandez-Gutiérrez, Salvador Pane, Bradley J. Nelson

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

46 Citations (Scopus)

Abstract

We present a luminescence oxygen sensor integrated with a wireless intraocular microrobot for minimally-invasive diagnosis. This microrobot can be accurately controlled in the intraocular cavity by applying magnetic fields. The microrobot consists of a magnetic body susceptible to magnetic fields and a sensor coating. This coating embodies Pt(II) octaethylporphine (PtOEP) dyes as the luminescence material and polystyrene as a supporting matrix, and it can be wirelessly excited and read out by optical means. The sensor works based on quenching of luminescence in the presence of oxygen. The excitation and emission spectrum, response time, and oxygen sensitivity of the sensor were characterized using a spectrometer. A custom device was designed and built to use this sensor for intraocular measurements with the microrobot. Due to the intrinsic nature of luminescence lifetimes, a frequency-domain lifetime measurement approach was used. An alternative sensor design with increased performance was demonstrated by using poly(styrene-co-maleic anhydride) (PS-MA) and PtOEP nanospheres.

Original languageEnglish
Article number6291753
Pages (from-to)3104-3109
Number of pages6
JournalIEEE Transactions on Biomedical Engineering
Volume59
Issue number12 PART2
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Keywords

  • Actuation
  • magnetic
  • microrobots
  • nanosphere
  • photoluminescence
  • porphine

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