TY - JOUR
T1 - Improved bioconversion of crude glycerol to hydrogen by statistical optimization of media components
AU - Mangayil, Rahul
AU - Aho, Tommi
AU - Karp, Matti
AU - Santala, Ville
N1 - Funding Information:
The research was funded by the Maj and Tor Nessling Foundation (Project no: 2012356 ) and The Academy of Finland (Project no's. 126974 and 139830 ).
Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2015/3/1
Y1 - 2015/3/1
N2 - Bioconversion of crude glycerol to hydrogen has gained importance as it addresses both sustainable energy production and waste disposal issues. Until recently, statistical optimizations of crude glycerol bioconversion to hydrogen have been greatly focused on pure strains. In this study, biohydrogen production from crude glycerol by an enriched microbial culture (predominated with Clostridium species) was improved by statistical optimization of media components. Plackett-Burman design identified MgCl2.6H2O and KCl with negative effect on hydrogen production and selected NH4Cl, K2HPO4 and KH2PO4 as significant variables. Box-Behnken design indicated the optimal region beyond design area and studies were continued by ridge analysis. Central composite face centered design envisaged a maximal hydrogen yield of 1.41mol-H2/mol-glycerolconsumed at concentrations 4.40g/L and 2.27g/L for NH4Cl and KH2PO4 respectively. Confirmation experiment with the optimized media (NH4Cl, 4.40g/L; K2HPO4, 1.6g/L; KH2PO4, 2.27g/L; MgCl2.6H2O, 1.0g/L; KCl, 1.0g/L; Na-acetate.3H2O, 1.0g/L and tryptone, 2.0g/L) revealed an excellent correlation between predicted and experimental hydrogen yield. Optimization of media components by design of experiments enhanced hydrogen yield by 29%.
AB - Bioconversion of crude glycerol to hydrogen has gained importance as it addresses both sustainable energy production and waste disposal issues. Until recently, statistical optimizations of crude glycerol bioconversion to hydrogen have been greatly focused on pure strains. In this study, biohydrogen production from crude glycerol by an enriched microbial culture (predominated with Clostridium species) was improved by statistical optimization of media components. Plackett-Burman design identified MgCl2.6H2O and KCl with negative effect on hydrogen production and selected NH4Cl, K2HPO4 and KH2PO4 as significant variables. Box-Behnken design indicated the optimal region beyond design area and studies were continued by ridge analysis. Central composite face centered design envisaged a maximal hydrogen yield of 1.41mol-H2/mol-glycerolconsumed at concentrations 4.40g/L and 2.27g/L for NH4Cl and KH2PO4 respectively. Confirmation experiment with the optimized media (NH4Cl, 4.40g/L; K2HPO4, 1.6g/L; KH2PO4, 2.27g/L; MgCl2.6H2O, 1.0g/L; KCl, 1.0g/L; Na-acetate.3H2O, 1.0g/L and tryptone, 2.0g/L) revealed an excellent correlation between predicted and experimental hydrogen yield. Optimization of media components by design of experiments enhanced hydrogen yield by 29%.
KW - Biohydrogen
KW - Crude glycerol
KW - Optimization
KW - Response surface methodology
UR - http://www.scopus.com/inward/record.url?scp=84910051633&partnerID=8YFLogxK
U2 - 10.1016/j.renene.2014.10.051
DO - 10.1016/j.renene.2014.10.051
M3 - Article
AN - SCOPUS:84910051633
SN - 0960-1481
VL - 75
SP - 583
EP - 589
JO - Renewable Energy
JF - Renewable Energy
ER -
