Immunoassays Based on Hot Electron-Induced Electrochemiluminescence at Disposable Cell Chips with Printed Electrodes

Päivi Grönroos, Nur Nur-E-Habiba, Kalle Salminen, Marja Nissinen, Tomi Tuomaala, Kim Miikki, Qiang Zhang, Nan Wei, Esko Kauppinen, Jarkko Eskola, Harri Härmä, Sakari Kulmala

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Abstract

Novel hot electron-emitting working electrodes and conventional counter electrodes were created by screen printing. Thus, low-cost disposable electrode chips for bioaffinity assays were produced to replace our older expensive electrode chips manufactured by manufacturing techniques of electronics from silicon or on glass chips. The present chips were created by printing as follows: (i) silver lines provided the electronic contacts, counter electrode and the bottom of the working electrode and counter electrode, (ii) the composite layer was printed on appropriate parts of the silver layer, and (iii) finally a hydrophobic ring was added to produce the electrochemical cell boundaries. The applicability of these electrode chips in bioaffinity assays was demonstrated by an immunoassay of human C-reactive protein (i) using Tb(III) chelate label displaying long-lived hot electron-induced electrochemiluminescence (HECL) and (ii) now for the first time fluorescein isothiocyanate (FITC) was utilized as an a low-cost organic label displaying a short-lived HECL in a real-world bioaffinity assay.

Original languageEnglish
Article number2751
Number of pages13
JournalSensors (Basel, Switzerland)
Volume19
Issue number12
DOIs
Publication statusPublished - 19 Jun 2019
MoE publication typeA1 Journal article-refereed

Keywords

  • bioaffinity assays
  • C-reactive protein
  • electrochemiluminescence
  • ethyl cellulose
  • fluorescein isothiocyanate
  • hot electron electrochemistry
  • polystyrene
  • screen printed electrodes
  • terbium chelate electrochemiluminescence

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