Immobilized cellulose nanospheres enable rapid antigen detection in lateral flow immunoassays

Katariina Solin; Marco Beaumont; Maryam Borghei; Hannes Orelma; Pascal Mertens; Orlando J. Rojas

doi:10.1007/s10570-022-05038-y

Immobilized cellulose nanospheres enable rapid antigen detection in lateral flow immunoassays

Katariina Solin, Marco Beaumont, Maryam Borghei, Hannes Orelma, Pascal Mertens, Orlando J. Rojas^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

5 Citations (Scopus)

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Abstract

Rapid diagnostic systems are essential in controlling the spread of viral pathogens and efficient patient management. The available technologies for low-cost viral antigen testing have several limitations, including a lack of accuracy and sensitivity. Here, we introduce a platform based on cellulose II nanoparticles (oppositely charged NPan and NPcat) for effective control of surface protein interactions, leading to rapid and sensitive antigen tests. Passivation against non-specific adsorption and augmented immobilization of sensing antibodies is achieved by adjusting the electrostatic charge of the nanoparticles. The interactions affecting the performance of the system are investigated by microgravimetry and confocal imaging. As a proof-of-concept test, SARS-CoV-2 nucleocapsid sensing was carried out by using saliva-wicking by channels that were stencil-printed on paper. We conclude that inkjet-printed NPcat elicits strong optical signals, visible after a few minutes, opening the opportunity for cost-effective and rapid diagnostic. Graphical abstract: [Figure not available: see fulltext.]

Original language	English
Pages (from-to)	2353-2365
Number of pages	13
Journal	Cellulose
Volume	30
Issue number	4
Early online date	5 Jan 2023
DOIs	https://doi.org/10.1007/s10570-022-05038-y
Publication status	Published - Mar 2023
MoE publication type	A1 Journal article-refereed

Keywords

Cellulose nanoparticles
Coronavirus antigen test
Immunoassays
Paper-based diagnostics
Patterning
Protein interactions

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/s10570-022-05038-yLicence: CC BY

CHEM_Solin_et_al_Immobilized_cellulose_nanospheres_2023_CelluloseFinal published version, 3.27 MBLicence: CC BY

Cite this

@article{aa42a3a4b5d043cfbbd102fc27d943db,

title = "Immobilized cellulose nanospheres enable rapid antigen detection in lateral flow immunoassays",

abstract = "Rapid diagnostic systems are essential in controlling the spread of viral pathogens and efficient patient management. The available technologies for low-cost viral antigen testing have several limitations, including a lack of accuracy and sensitivity. Here, we introduce a platform based on cellulose II nanoparticles (oppositely charged NPan and NPcat) for effective control of surface protein interactions, leading to rapid and sensitive antigen tests. Passivation against non-specific adsorption and augmented immobilization of sensing antibodies is achieved by adjusting the electrostatic charge of the nanoparticles. The interactions affecting the performance of the system are investigated by microgravimetry and confocal imaging. As a proof-of-concept test, SARS-CoV-2 nucleocapsid sensing was carried out by using saliva-wicking by channels that were stencil-printed on paper. We conclude that inkjet-printed NPcat elicits strong optical signals, visible after a few minutes, opening the opportunity for cost-effective and rapid diagnostic. Graphical abstract: [Figure not available: see fulltext.]",

keywords = "Cellulose nanoparticles, Coronavirus antigen test, Immunoassays, Paper-based diagnostics, Patterning, Protein interactions",

author = "Katariina Solin and Marco Beaumont and Maryam Borghei and Hannes Orelma and Pascal Mertens and Rojas, {Orlando J.}",

note = "| openaire: EC/H2020/760876/EU//INNPAPER | openaire: EC/H2020/788489/EU//BioELCell Funding Information: Authors acknowledge funding by the European Union{\textquoteright}s Horizon 2020 research and innovation programme under grant agreement No 760876. Also, funding by Academy of Finland's Flagship Programme under Projects Nos. 318890 and 318891 (Competence Center for Materials Bioeconomy, FinnCERES) is acknowledged. OJR is grateful for the support received from the ERC Advanced Grant Agreement No. 788489 ({"}BioElCell{"}) and The Canada Excellence Research Chair Program (CERC-2018-00006), as well as Canada Foundation for Innovation (Project Number 38623). KS acknowledges funding by the Aalto University School of Chemical Engineering doctoral programme. Dr. Dev Sriranganadane is acknowledged for his helpful discussion. Prof. Patrick Gane is acknowledged for his help in the development of the printed fluidic channel. We acknowledge the provision of facilities and technical support by Aalto University at OtaNano—Nanomicroscopy Center (Aalto-NMC). Funding Information: Open Access funding provided by Aalto University. This project was funded by the European Union{\textquoteright}s Horizon 2020 research and innovation programme under grant agreement No 760876. This work was a part of the Academy of Finland's Flagship Programme under Projects Nos. 318890 and 318891 (Competence Center for Materials Bioeconomy, FinnCERES). OJR acknowledges support from the ERC Advanced Grant Agreement No. 788489 ({"}BioElCell{"}) and The Canada Excellence Research Chair Program (CERC-2018-00006), as well as Canada Foundation for Innovation (Project Number 38623). KS acknowledges funding by the Aalto University School of Chemical Engineering doctoral programme. Publisher Copyright: {\textcopyright} 2023, The Author(s).",

year = "2023",

month = mar,

doi = "10.1007/s10570-022-05038-y",

language = "English",

volume = "30",

pages = "2353--2365",

journal = "Cellulose",

issn = "0969-0239",

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TY - JOUR

T1 - Immobilized cellulose nanospheres enable rapid antigen detection in lateral flow immunoassays

AU - Solin, Katariina

AU - Beaumont, Marco

AU - Borghei, Maryam

AU - Orelma, Hannes

AU - Mertens, Pascal

AU - Rojas, Orlando J.

N1 - | openaire: EC/H2020/760876/EU//INNPAPER | openaire: EC/H2020/788489/EU//BioELCell Funding Information: Authors acknowledge funding by the European Union’s Horizon 2020 research and innovation programme under grant agreement No 760876. Also, funding by Academy of Finland's Flagship Programme under Projects Nos. 318890 and 318891 (Competence Center for Materials Bioeconomy, FinnCERES) is acknowledged. OJR is grateful for the support received from the ERC Advanced Grant Agreement No. 788489 ("BioElCell") and The Canada Excellence Research Chair Program (CERC-2018-00006), as well as Canada Foundation for Innovation (Project Number 38623). KS acknowledges funding by the Aalto University School of Chemical Engineering doctoral programme. Dr. Dev Sriranganadane is acknowledged for his helpful discussion. Prof. Patrick Gane is acknowledged for his help in the development of the printed fluidic channel. We acknowledge the provision of facilities and technical support by Aalto University at OtaNano—Nanomicroscopy Center (Aalto-NMC). Funding Information: Open Access funding provided by Aalto University. This project was funded by the European Union’s Horizon 2020 research and innovation programme under grant agreement No 760876. This work was a part of the Academy of Finland's Flagship Programme under Projects Nos. 318890 and 318891 (Competence Center for Materials Bioeconomy, FinnCERES). OJR acknowledges support from the ERC Advanced Grant Agreement No. 788489 ("BioElCell") and The Canada Excellence Research Chair Program (CERC-2018-00006), as well as Canada Foundation for Innovation (Project Number 38623). KS acknowledges funding by the Aalto University School of Chemical Engineering doctoral programme. Publisher Copyright: © 2023, The Author(s).

PY - 2023/3

Y1 - 2023/3

N2 - Rapid diagnostic systems are essential in controlling the spread of viral pathogens and efficient patient management. The available technologies for low-cost viral antigen testing have several limitations, including a lack of accuracy and sensitivity. Here, we introduce a platform based on cellulose II nanoparticles (oppositely charged NPan and NPcat) for effective control of surface protein interactions, leading to rapid and sensitive antigen tests. Passivation against non-specific adsorption and augmented immobilization of sensing antibodies is achieved by adjusting the electrostatic charge of the nanoparticles. The interactions affecting the performance of the system are investigated by microgravimetry and confocal imaging. As a proof-of-concept test, SARS-CoV-2 nucleocapsid sensing was carried out by using saliva-wicking by channels that were stencil-printed on paper. We conclude that inkjet-printed NPcat elicits strong optical signals, visible after a few minutes, opening the opportunity for cost-effective and rapid diagnostic. Graphical abstract: [Figure not available: see fulltext.]

AB - Rapid diagnostic systems are essential in controlling the spread of viral pathogens and efficient patient management. The available technologies for low-cost viral antigen testing have several limitations, including a lack of accuracy and sensitivity. Here, we introduce a platform based on cellulose II nanoparticles (oppositely charged NPan and NPcat) for effective control of surface protein interactions, leading to rapid and sensitive antigen tests. Passivation against non-specific adsorption and augmented immobilization of sensing antibodies is achieved by adjusting the electrostatic charge of the nanoparticles. The interactions affecting the performance of the system are investigated by microgravimetry and confocal imaging. As a proof-of-concept test, SARS-CoV-2 nucleocapsid sensing was carried out by using saliva-wicking by channels that were stencil-printed on paper. We conclude that inkjet-printed NPcat elicits strong optical signals, visible after a few minutes, opening the opportunity for cost-effective and rapid diagnostic. Graphical abstract: [Figure not available: see fulltext.]

KW - Cellulose nanoparticles

KW - Coronavirus antigen test

KW - Immunoassays

KW - Paper-based diagnostics

KW - Patterning

KW - Protein interactions

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DO - 10.1007/s10570-022-05038-y

M3 - Article

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SN - 0969-0239

VL - 30

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EP - 2365

JO - Cellulose

JF - Cellulose

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ER -

Immobilized cellulose nanospheres enable rapid antigen detection in lateral flow immunoassays

Abstract

Keywords

UN SDGs

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Other files and links

Fingerprint

BioELCell: Bioproducts Engineered from Lignocelluloses: from plants and upcycling to next generation materials

FinnCERES: Competence Center for the Materials Bioeconomy: A Flagship for our Sustainable Future

INNPAPER: Innovative and Smart Printed Electronics based on Multifunctionalized Paper: from Smart Labelling to Point of Care Bioplatforms

OtaNano

OtaNano - Nanomicroscopy Center

Cite this

Immobilized cellulose nanospheres enable rapid antigen detection in lateral flow immunoassays

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Projects

BioELCell: Bioproducts Engineered from Lignocelluloses: from plants and upcycling to next generation materials

FinnCERES: Competence Center for the Materials Bioeconomy: A Flagship for our Sustainable Future

INNPAPER: Innovative and Smart Printed Electronics based on Multifunctionalized Paper: from Smart Labelling to Point of Care Bioplatforms

Equipment

OtaNano

OtaNano - Nanomicroscopy Center

Cite this