Immobilization of membrane-bound enzymes on micropillar arrays via fusogenic liposomes

Iiro Kiiski; Tea Pihlaja; Lauri Urvas; Ville Jokinen; Tiina Sikanen

Immobilization of membrane-bound enzymes on micropillar arrays via fusogenic liposomes

Iiro Kiiski^*
, Tea Pihlaja
, Lauri Urvas
, Ville Jokinen
, Tiina Sikanen

^*Corresponding author for this work

University of Helsinki

Research output: Contribution to conference › Abstract › Scientific › peer-review

Abstract

This work reports a new immobilization strategy for membrane-bound enzymes, such as the cytochromes P450 (CYPs), the major class of drug metabolizing enzymes. Our immobilization method relies on natural biotinylation of the lipid membrane with the help of fusogenic liposomes. With our method, CYPs (both human and recombinant) can be immobilized on streptavidin-functionalized micropillar arrays without affecting the enzyme kinetic parameters or stability, which has been challenging to most of the previously reported approaches, such as physical entrapment or covalent bonding of the enzymes.

Original language	English
Pages	2121-2123
Number of pages	3
Publication status	Published - 2018
MoE publication type	Not Eligible
Event	International Conference on Miniaturized Systems for Chemistry and Life Sciences - Kaohsiung, Taiwan, Republic of China Duration: 11 Nov 2018 → 15 Nov 2018 Conference number: 22

Conference

Conference	International Conference on Miniaturized Systems for Chemistry and Life Sciences
Abbreviated title	MicroTAS
Country/Territory	Taiwan, Republic of China
City	Kaohsiung
Period	11/11/2018 → 15/11/2018

Funding

This work was financially supported by the European Research Council under the European Union's Seventh Framework Programme (FP/2007-2013)/ ERC Grant Agreement no. 311705 (CUMTAS). The work was also supported by the Academy of Finland (grants no. 304400, 314303, 297360), the University of Helsinki Research Funds, and the DPDR doctoral programme, University of Helsinki. Magnus Ehrnrooth foundation is also acknowledged for travel support.

Keywords

Cytochrome P450
Drug metabolism
Enzyme immobilization
Enzyme microreactors

Cite this

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title = "Immobilization of membrane-bound enzymes on micropillar arrays via fusogenic liposomes",

abstract = "This work reports a new immobilization strategy for membrane-bound enzymes, such as the cytochromes P450 (CYPs), the major class of drug metabolizing enzymes. Our immobilization method relies on natural biotinylation of the lipid membrane with the help of fusogenic liposomes. With our method, CYPs (both human and recombinant) can be immobilized on streptavidin-functionalized micropillar arrays without affecting the enzyme kinetic parameters or stability, which has been challenging to most of the previously reported approaches, such as physical entrapment or covalent bonding of the enzymes.",

keywords = "Cytochrome P450, Drug metabolism, Enzyme immobilization, Enzyme microreactors",

author = "Iiro Kiiski and Tea Pihlaja and Lauri Urvas and Ville Jokinen and Tiina Sikanen",

year = "2018",

language = "English",

pages = "2121--2123",

note = "International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS ; Conference date: 11-11-2018 Through 15-11-2018",

}

Immobilization of membrane-bound enzymes on micropillar arrays via fusogenic liposomes. / Kiiski, Iiro; Pihlaja, Tea; Urvas, Lauri et al.
2018. 2121-2123 Abstract from International Conference on Miniaturized Systems for Chemistry and Life Sciences, Kaohsiung, Taiwan, Republic of China.

Research output: Contribution to conference › Abstract › Scientific › peer-review

TY - CONF

T1 - Immobilization of membrane-bound enzymes on micropillar arrays via fusogenic liposomes

AU - Kiiski, Iiro

AU - Pihlaja, Tea

AU - Urvas, Lauri

AU - Jokinen, Ville

AU - Sikanen, Tiina

N1 - Conference code: 22

PY - 2018

Y1 - 2018

N2 - This work reports a new immobilization strategy for membrane-bound enzymes, such as the cytochromes P450 (CYPs), the major class of drug metabolizing enzymes. Our immobilization method relies on natural biotinylation of the lipid membrane with the help of fusogenic liposomes. With our method, CYPs (both human and recombinant) can be immobilized on streptavidin-functionalized micropillar arrays without affecting the enzyme kinetic parameters or stability, which has been challenging to most of the previously reported approaches, such as physical entrapment or covalent bonding of the enzymes.

AB - This work reports a new immobilization strategy for membrane-bound enzymes, such as the cytochromes P450 (CYPs), the major class of drug metabolizing enzymes. Our immobilization method relies on natural biotinylation of the lipid membrane with the help of fusogenic liposomes. With our method, CYPs (both human and recombinant) can be immobilized on streptavidin-functionalized micropillar arrays without affecting the enzyme kinetic parameters or stability, which has been challenging to most of the previously reported approaches, such as physical entrapment or covalent bonding of the enzymes.

KW - Cytochrome P450

KW - Drug metabolism

KW - Enzyme immobilization

KW - Enzyme microreactors

UR - https://www.scopus.com/pages/publications/85079811695

M3 - Abstract

AN - SCOPUS:85079811695

SP - 2121

EP - 2123

T2 - International Conference on Miniaturized Systems for Chemistry and Life Sciences

Y2 - 11 November 2018 through 15 November 2018

ER -

Immobilization of membrane-bound enzymes on micropillar arrays via fusogenic liposomes

Abstract

Conference

Funding

Keywords

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