Lactobacillus rhamnosus GG and its SpaC pilus adhesin modulate inflammatory responsiveness and TLR-related gene expression in the fetal human gut

Kriston Ganguli; Maria C. Collado; Jaana Rautava; Lei Lu; Reetta Satokari; Ingemar von Ossowski; Justus Reunanen; Willem M. de Vos; Airi Palva; Erika Isolauri; Seppo Salminen; W. Allan Walker; Samuli Rautava

doi:10.1038/pr.2015.5

Lactobacillus rhamnosus GG and its SpaC pilus adhesin modulate inflammatory responsiveness and TLR-related gene expression in the fetal human gut

Kriston Ganguli, Maria C. Collado, Jaana Rautava, Lei Lu, Reetta Satokari, Ingemar von Ossowski, Justus Reunanen, Willem M. de Vos, Airi Palva, Erika Isolauri, Seppo Salminen, W. Allan Walker, Samuli Rautava^*

^*Corresponding author for this work

Not published at Aalto University

Research output: Contribution to journal › Article › Scientific › peer-review

Abstract

BACKGROUND: Bacterial contact in utero modulates fetal and neonatal immune responses. Maternal probiotic supplementation reduces the risk of immune-mediated disease in the infant. We investigated the immunomodulatory properties of live Lactobacillus rhamnosus GG and its SpaC pilus adhesin in human fetal intestinal models.

METHODS: Tumor necrosis factor (TNF)-alpha mRNA expression was measured by qPCR in a human fetal intestinal organ culture model exposed to live Lactobacillus rhamnosus GG and proinflammatory stimuli. Binding of recombinant SpaC pilus protein to intestinal epithelial cells (IECs) was assessed in human fetal intestinal organ culture and the human fetal intestinal epithelial cell line H4 by immunohistochemistry and immunofluorescence, respectively. TLR-related gene expression in fetal ileal organ culture after exposure to recombinant SpaC was assessed by qPCR.

RESULTS: Live Lactobacillus rhamnosus GG significantly attenuates pathogen-induced TNF-alpha mRNA expression in the human fetal gut. Recombinant SpaC protein was found to adhere to the fetal gut and to modulate varying levels of TLR-related gene expression.

CONCLUSION: The human fetal gut is responsive to luminal microbes. Lactobacillus rhamnosus GG significantly attenuates fetal intestinal inflammatory responses to pathogenic bacteria. The Lactobacillus rhamnosus GG pilus adhesin SpaC binds to immature human IECs and directly modulates IEC innate immune gene expression.

Original language	English
Pages (from-to)	528-535
Number of pages	8
Journal	Pediatric Research
Volume	77
Issue number	4
DOIs	https://doi.org/10.1038/pr.2015.5
Publication status	Published - Apr 2015
MoE publication type	A1 Journal article-refereed

Keywords

NECROTIZING ENTEROCOLITIS
PREGNANCY
PROBIOTICS
CELLS
MICROBIOME
PLACENTA
BACTERIA
DISEASE

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/pr.2015.5

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Ganguli, K., Collado, M. C., Rautava, J., Lu, L., Satokari, R., von Ossowski, I., Reunanen, J., de Vos, W. M., Palva, A., Isolauri, E., Salminen, S., Walker, W. A., & Rautava, S. (2015). Lactobacillus rhamnosus GG and its SpaC pilus adhesin modulate inflammatory responsiveness and TLR-related gene expression in the fetal human gut. Pediatric Research, 77(4), 528-535. https://doi.org/10.1038/pr.2015.5

@article{c5cd3435dbe04c30972a4d6e9436214a,

title = "Lactobacillus rhamnosus GG and its SpaC pilus adhesin modulate inflammatory responsiveness and TLR-related gene expression in the fetal human gut",

abstract = "BACKGROUND: Bacterial contact in utero modulates fetal and neonatal immune responses. Maternal probiotic supplementation reduces the risk of immune-mediated disease in the infant. We investigated the immunomodulatory properties of live Lactobacillus rhamnosus GG and its SpaC pilus adhesin in human fetal intestinal models.METHODS: Tumor necrosis factor (TNF)-alpha mRNA expression was measured by qPCR in a human fetal intestinal organ culture model exposed to live Lactobacillus rhamnosus GG and proinflammatory stimuli. Binding of recombinant SpaC pilus protein to intestinal epithelial cells (IECs) was assessed in human fetal intestinal organ culture and the human fetal intestinal epithelial cell line H4 by immunohistochemistry and immunofluorescence, respectively. TLR-related gene expression in fetal ileal organ culture after exposure to recombinant SpaC was assessed by qPCR.RESULTS: Live Lactobacillus rhamnosus GG significantly attenuates pathogen-induced TNF-alpha mRNA expression in the human fetal gut. Recombinant SpaC protein was found to adhere to the fetal gut and to modulate varying levels of TLR-related gene expression.CONCLUSION: The human fetal gut is responsive to luminal microbes. Lactobacillus rhamnosus GG significantly attenuates fetal intestinal inflammatory responses to pathogenic bacteria. The Lactobacillus rhamnosus GG pilus adhesin SpaC binds to immature human IECs and directly modulates IEC innate immune gene expression.",

keywords = "NECROTIZING ENTEROCOLITIS, PREGNANCY, PROBIOTICS, CELLS, MICROBIOME, PLACENTA, BACTERIA, DISEASE",

author = "Kriston Ganguli and Collado, {Maria C.} and Jaana Rautava and Lei Lu and Reetta Satokari and {von Ossowski}, Ingemar and Justus Reunanen and {de Vos}, {Willem M.} and Airi Palva and Erika Isolauri and Seppo Salminen and Walker, {W. Allan} and Samuli Rautava",

year = "2015",

month = apr,

doi = "10.1038/pr.2015.5",

language = "English",

volume = "77",

pages = "528--535",

journal = "Pediatric Research",

issn = "0031-3998",

publisher = "Lippincott Williams & Wilkins",

number = "4",

}

Ganguli, K, Collado, MC, Rautava, J, Lu, L, Satokari, R, von Ossowski, I, Reunanen, J, de Vos, WM, Palva, A, Isolauri, E, Salminen, S, Walker, WA & Rautava, S 2015, 'Lactobacillus rhamnosus GG and its SpaC pilus adhesin modulate inflammatory responsiveness and TLR-related gene expression in the fetal human gut', Pediatric Research, vol. 77, no. 4, pp. 528-535. https://doi.org/10.1038/pr.2015.5

TY - JOUR

T1 - Lactobacillus rhamnosus GG and its SpaC pilus adhesin modulate inflammatory responsiveness and TLR-related gene expression in the fetal human gut

AU - Ganguli, Kriston

AU - Collado, Maria C.

AU - Rautava, Jaana

AU - Lu, Lei

AU - Satokari, Reetta

AU - von Ossowski, Ingemar

AU - Reunanen, Justus

AU - de Vos, Willem M.

AU - Palva, Airi

AU - Isolauri, Erika

AU - Salminen, Seppo

AU - Walker, W. Allan

AU - Rautava, Samuli

PY - 2015/4

Y1 - 2015/4

N2 - BACKGROUND: Bacterial contact in utero modulates fetal and neonatal immune responses. Maternal probiotic supplementation reduces the risk of immune-mediated disease in the infant. We investigated the immunomodulatory properties of live Lactobacillus rhamnosus GG and its SpaC pilus adhesin in human fetal intestinal models.METHODS: Tumor necrosis factor (TNF)-alpha mRNA expression was measured by qPCR in a human fetal intestinal organ culture model exposed to live Lactobacillus rhamnosus GG and proinflammatory stimuli. Binding of recombinant SpaC pilus protein to intestinal epithelial cells (IECs) was assessed in human fetal intestinal organ culture and the human fetal intestinal epithelial cell line H4 by immunohistochemistry and immunofluorescence, respectively. TLR-related gene expression in fetal ileal organ culture after exposure to recombinant SpaC was assessed by qPCR.RESULTS: Live Lactobacillus rhamnosus GG significantly attenuates pathogen-induced TNF-alpha mRNA expression in the human fetal gut. Recombinant SpaC protein was found to adhere to the fetal gut and to modulate varying levels of TLR-related gene expression.CONCLUSION: The human fetal gut is responsive to luminal microbes. Lactobacillus rhamnosus GG significantly attenuates fetal intestinal inflammatory responses to pathogenic bacteria. The Lactobacillus rhamnosus GG pilus adhesin SpaC binds to immature human IECs and directly modulates IEC innate immune gene expression.

AB - BACKGROUND: Bacterial contact in utero modulates fetal and neonatal immune responses. Maternal probiotic supplementation reduces the risk of immune-mediated disease in the infant. We investigated the immunomodulatory properties of live Lactobacillus rhamnosus GG and its SpaC pilus adhesin in human fetal intestinal models.METHODS: Tumor necrosis factor (TNF)-alpha mRNA expression was measured by qPCR in a human fetal intestinal organ culture model exposed to live Lactobacillus rhamnosus GG and proinflammatory stimuli. Binding of recombinant SpaC pilus protein to intestinal epithelial cells (IECs) was assessed in human fetal intestinal organ culture and the human fetal intestinal epithelial cell line H4 by immunohistochemistry and immunofluorescence, respectively. TLR-related gene expression in fetal ileal organ culture after exposure to recombinant SpaC was assessed by qPCR.RESULTS: Live Lactobacillus rhamnosus GG significantly attenuates pathogen-induced TNF-alpha mRNA expression in the human fetal gut. Recombinant SpaC protein was found to adhere to the fetal gut and to modulate varying levels of TLR-related gene expression.CONCLUSION: The human fetal gut is responsive to luminal microbes. Lactobacillus rhamnosus GG significantly attenuates fetal intestinal inflammatory responses to pathogenic bacteria. The Lactobacillus rhamnosus GG pilus adhesin SpaC binds to immature human IECs and directly modulates IEC innate immune gene expression.

KW - NECROTIZING ENTEROCOLITIS

KW - PREGNANCY

KW - PROBIOTICS

KW - CELLS

KW - MICROBIOME

KW - PLACENTA

KW - BACTERIA

KW - DISEASE

U2 - 10.1038/pr.2015.5

DO - 10.1038/pr.2015.5

M3 - Article

SN - 0031-3998

VL - 77

SP - 528

EP - 535

JO - Pediatric Research

JF - Pediatric Research

IS - 4

ER -

Lactobacillus rhamnosus GG and its SpaC pilus adhesin modulate inflammatory responsiveness and TLR-related gene expression in the fetal human gut

Abstract

Keywords

UN SDGs

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