Identification of the cellulose synthase genes from the Oomycete Saprolegnia monoica and effect of cellulose synthesis inhibitors on gene expression and enzyme activity

Johanna Fugelstad, Jamel Bouzenzana, Soraya Djerbi, Gea Guerriero, Ines Ezcurra, Tuula T. Teeri, Lars Arvestad, Vincent Bulone*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Cellulose biosynthesis is a vital but yet poorly understood biochemical process in Oomycetes. Here, we report the identification and characterization of the cellulose synthase genes (CesA) from Saprolegnia monoica. Southern blot experiments revealed the occurrence of three CesA homologues in this species and phylogenetic analyses confirmed that Oomycete CesAs form a clade of their own. All gene products contained the D,D,D,QXXRW signature of most processive glycosyltransferases, including cellulose synthases. However, their N-terminal ends exhibited Oomycete-specific domains, i.e. Pleckstrin Homology domains, or conserved domains of an unknown function together with additional putative transmembrane domains. Mycelial growth was inhibited in the presence of the cellulose biosynthesis inhibitors 2,6-dichlorobenzonitrile or Congo Red. This inhibition was accompanied by a higher expression of all CesA genes in the mycelium and increased in vitro glucan synthase activities. Altogether, our data strongly suggest a direct involvement of the identified CesA genes in cellulose biosynthesis. (C) 2009 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)759-767
Number of pages9
JournalFungal Genetics and Biology
Volume46
Issue number10
DOIs
Publication statusPublished - Oct 2009
MoE publication typeA1 Journal article-refereed

Keywords

  • Cellulose synthase genes
  • Cell wall biosynthesis
  • Congo Red
  • 2,6-Dichlorobenzonitrile (DCB)
  • Oomycetes
  • Saprolegnia monoica
  • FUNGUS SAPROLEGNIA
  • ACETOBACTER-XYLINUM
  • CATALYTIC SUBUNIT
  • CHITIN SYNTHASE
  • PLASMA-MEMBRANE
  • WALL CHEMISTRY
  • CELL-WALLS
  • CONGO RED
  • BIOSYNTHESIS
  • DNA

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