Identification in the mould Hypocrea jecorina of a gene encoding an NADP(+): D-xylose dehydrogenase

Suvi Berghäll, Satu Hilditch, Merja Penttilä, Peter Richard*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

A gene coding for an NADP(+)-dependent D-xylose dehydrogenase was identified in the mould Hypocrea jecorina (Trichoderma reesei). It was cloned from cDNA, the active enzyme was expressed in yeast and a histidine-tagged enzyme was purified and characterized. The enzyme had highest activity with D-xylose and significantly smaller activities with other aldose sugars. The enzyme is specific for NADP(+). The K-m values for D-xylose and NADP(+) are 43 mM and 250 mu M, respectively. The role of this enzyme in H. jecorina is unclear because in this organism D-xylose is predominantly catabolized through a path with xylitol and D-xylulose as intermediates and the mould is unable to grow on D-xylonic acid.

Original languageEnglish
Pages (from-to)249-253
Number of pages5
JournalFEMS Microbiology Letters
Volume277
Issue number2
DOIs
Publication statusPublished - Dec 2007
MoE publication typeA1 Journal article-refereed

Keywords

  • D-xylose dehydrogenase
  • D-xylose metabolism
  • Hypocrea jecorina
  • Trichoderma reesei
  • NADP-linked D-xylose dehydrogenase
  • EC 1.1.1.175
  • L-ARABINOSE METABOLISM
  • CELL-FREE-EXTRACTS
  • SACCHAROMYCES-CEREVISIAE
  • ALTERNATIVE PATHWAY
  • PIG-LIVER
  • EXPRESSION
  • OXIDATION
  • CLONING
  • PURIFICATION
  • DEHYDRATASE

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