Identification in the mould Hypocrea jecorina of a gene encoding an NADP(+): D-xylose dehydrogenase

Suvi Berghäll; Satu Hilditch; Merja Penttilä; Peter Richard

doi:10.1111/j.1574-6968.2007.00969.x

Identification in the mould Hypocrea jecorina of a gene encoding an NADP(+): D-xylose dehydrogenase

Suvi Berghäll, Satu Hilditch, Merja Penttilä, Peter Richard^*

^*Corresponding author for this work

Not published at Aalto University

Research output: Contribution to journal › Article › Scientific › peer-review

Abstract

A gene coding for an NADP(+)-dependent D-xylose dehydrogenase was identified in the mould Hypocrea jecorina (Trichoderma reesei). It was cloned from cDNA, the active enzyme was expressed in yeast and a histidine-tagged enzyme was purified and characterized. The enzyme had highest activity with D-xylose and significantly smaller activities with other aldose sugars. The enzyme is specific for NADP(+). The K-m values for D-xylose and NADP(+) are 43 mM and 250 mu M, respectively. The role of this enzyme in H. jecorina is unclear because in this organism D-xylose is predominantly catabolized through a path with xylitol and D-xylulose as intermediates and the mould is unable to grow on D-xylonic acid.

Original language	English
Pages (from-to)	249-253
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	277
Issue number	2
DOIs	https://doi.org/10.1111/j.1574-6968.2007.00969.x
Publication status	Published - Dec 2007
MoE publication type	A1 Journal article-refereed

Keywords

D-xylose dehydrogenase
D-xylose metabolism
Hypocrea jecorina
Trichoderma reesei
NADP-linked D-xylose dehydrogenase
EC 1.1.1.175
L-ARABINOSE METABOLISM
CELL-FREE-EXTRACTS
SACCHAROMYCES-CEREVISIAE
ALTERNATIVE PATHWAY
PIG-LIVER
EXPRESSION
OXIDATION
CLONING
PURIFICATION
DEHYDRATASE

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@article{8eff324548e248cfbf860fc5f7dbe8a9,

title = "Identification in the mould Hypocrea jecorina of a gene encoding an NADP(+): D-xylose dehydrogenase",

abstract = "A gene coding for an NADP(+)-dependent D-xylose dehydrogenase was identified in the mould Hypocrea jecorina (Trichoderma reesei). It was cloned from cDNA, the active enzyme was expressed in yeast and a histidine-tagged enzyme was purified and characterized. The enzyme had highest activity with D-xylose and significantly smaller activities with other aldose sugars. The enzyme is specific for NADP(+). The K-m values for D-xylose and NADP(+) are 43 mM and 250 mu M, respectively. The role of this enzyme in H. jecorina is unclear because in this organism D-xylose is predominantly catabolized through a path with xylitol and D-xylulose as intermediates and the mould is unable to grow on D-xylonic acid.",

keywords = "D-xylose dehydrogenase, D-xylose metabolism, Hypocrea jecorina, Trichoderma reesei, NADP-linked D-xylose dehydrogenase, EC 1.1.1.175, L-ARABINOSE METABOLISM, CELL-FREE-EXTRACTS, SACCHAROMYCES-CEREVISIAE, ALTERNATIVE PATHWAY, PIG-LIVER, EXPRESSION, OXIDATION, CLONING, PURIFICATION, DEHYDRATASE",

author = "Suvi Bergh{\"a}ll and Satu Hilditch and Merja Penttil{\"a} and Peter Richard",

year = "2007",

month = dec,

doi = "10.1111/j.1574-6968.2007.00969.x",

language = "English",

volume = "277",

pages = "249--253",

journal = "FEMS Microbiology Letters",

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number = "2",

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TY - JOUR

T1 - Identification in the mould Hypocrea jecorina of a gene encoding an NADP(+)

T2 - D-xylose dehydrogenase

AU - Berghäll, Suvi

AU - Hilditch, Satu

AU - Penttilä, Merja

AU - Richard, Peter

PY - 2007/12

Y1 - 2007/12

N2 - A gene coding for an NADP(+)-dependent D-xylose dehydrogenase was identified in the mould Hypocrea jecorina (Trichoderma reesei). It was cloned from cDNA, the active enzyme was expressed in yeast and a histidine-tagged enzyme was purified and characterized. The enzyme had highest activity with D-xylose and significantly smaller activities with other aldose sugars. The enzyme is specific for NADP(+). The K-m values for D-xylose and NADP(+) are 43 mM and 250 mu M, respectively. The role of this enzyme in H. jecorina is unclear because in this organism D-xylose is predominantly catabolized through a path with xylitol and D-xylulose as intermediates and the mould is unable to grow on D-xylonic acid.

AB - A gene coding for an NADP(+)-dependent D-xylose dehydrogenase was identified in the mould Hypocrea jecorina (Trichoderma reesei). It was cloned from cDNA, the active enzyme was expressed in yeast and a histidine-tagged enzyme was purified and characterized. The enzyme had highest activity with D-xylose and significantly smaller activities with other aldose sugars. The enzyme is specific for NADP(+). The K-m values for D-xylose and NADP(+) are 43 mM and 250 mu M, respectively. The role of this enzyme in H. jecorina is unclear because in this organism D-xylose is predominantly catabolized through a path with xylitol and D-xylulose as intermediates and the mould is unable to grow on D-xylonic acid.

KW - D-xylose dehydrogenase

KW - D-xylose metabolism

KW - Hypocrea jecorina

KW - Trichoderma reesei

KW - NADP-linked D-xylose dehydrogenase

KW - EC 1.1.1.175

KW - L-ARABINOSE METABOLISM

KW - CELL-FREE-EXTRACTS

KW - SACCHAROMYCES-CEREVISIAE

KW - ALTERNATIVE PATHWAY

KW - PIG-LIVER

KW - EXPRESSION

KW - OXIDATION

KW - CLONING

KW - PURIFICATION

KW - DEHYDRATASE

U2 - 10.1111/j.1574-6968.2007.00969.x

DO - 10.1111/j.1574-6968.2007.00969.x

M3 - Article

VL - 277

SP - 249

EP - 253

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2

ER -

Identification in the mould Hypocrea jecorina of a gene encoding an NADP(+): D-xylose dehydrogenase

Abstract

Keywords

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