Hydroperoxide production from linoleic acid by heterologous Gaeumannomyces graminis tritici lipoxygenase: Optimization and scale-up

Research output: Contribution to journalArticle

Researchers

  • Juan José Villaverde
  • Vincent van der Vlist
  • Sónia A.O. Santos
  • Thomas Haarmann
  • Kim Langfelder
  • Minni Pirttimaa
  • Antti Nyyssölä
  • Sirpa Jylhä
  • Tarja Tamminen
  • Kristiina Kruus

  • Leo de Graaff
  • Carlos Pascoal Neto
  • Mário M.Q. Simões
  • M. R.M. Domingues
  • Armando J.D. Silvestre
  • Jasmin Eidner
  • Johanna Buchert

Research units

  • University of Aveiro
  • Wageningen University & Research
  • Associated British Foods
  • VTT Technical Research Centre of Finland

Abstract

Linoleic acid was converted into hydroperoxides by a G. aeumannomyces graminis tritici lipoxygenase produced recombinantly in Trichoderma reesei. Hydroperoxide production was optimized using a face-centred experimental design in order to study the effects of pH, temperature and time on the conversion of linoleic acid into four regioisomeric hydroperoxyoctadecadienoic acids (HPODE): 13-(Z,E)-, 9-(E,Z)-, 13-(E,E)-, 9-(E,E)-HPODE. Fitting equations described satisfactorily the system behavior and showed that reaction time was the most influencing independent variable. A set of independent variables (pH = 6.7, temperature = 23.9 °C and time = 18. h) allowed to obtain high yields of hydroperoxides (88.0%) with a good selectivity for the 13-(Z,E)-HPODE isomer (47.4%) when the initial substrate concentration was 10. g/L. The production was further investigated using industrially relevant linoleic acid concentrations (100-300. g/L) leading to HPODE yields of ∼40% and the volumetric productivity 3.6. g/(L. h), and a selectivity for 13-(Z,E)-HPODE of around 74%.

Details

Original languageEnglish
Pages (from-to)82-90
Number of pages9
JournalChemical Engineering Journal
Volume217
Publication statusPublished - 1 Feb 2013
MoE publication typeA1 Journal article-refereed

    Research areas

  • Fatty acid hydroperoxides, Linoleic acid, Lipoxygenase, Optimization, Scale-up

ID: 32944463