Hybrid membrane biomaterials from self-assembly in polysaccharide and peptide amphiphile mixtures: controllable structural and mechanical properties and antimicrobial activity

Research output: Contribution to journalArticleScientificpeer-review

Researchers

  • V. Castelletto
  • A. Kaur
  • I. W. Hamley
  • R. H. Barnes
  • K. A. Karatzas
  • D. Hermida-Merino
  • S. Swioklo
  • C. J. Connon
  • J. Stasiak
  • M. Reza
  • Janne Ruokolainen

Research units

  • University of Reading
  • ESRF-The European Synchrotron
  • Newcastle University Business School
  • University of Cambridge

Abstract

Macroscopic capsules, with tunable properties based on hierarchical self-assembly on multiple lengthscales, are prepared from the co-operative self-assembly of polysaccharide and peptide amphiphiles. Different formulations can be used to create flexible membrane sacs in solution, soft capsules or rigid free-standing capsules. Samples are prepared by injecting a solution containing sodium alginate, with or without graphene oxide (GO), into a matrix consisting of a solution containing the peptide amphiphile PA C16-KKFF (K: lysine, F: phenylalanine), with or without CaCl2. Graphene oxide is added to the hybrid materials to modulate the mechanical properties of the capsules. Injection of sodium alginate solution into a pure PA matrix provides a flexible membrane sac in solution, while injection of NaAlg/GO solution into a PA matrix gives a soft capsule. Alternatively, a rigid free-standing capsule is made by injecting a NaAlg/GO solution into a PA + CaCl2 matrix solution. A comprehensive insight into the hierarchical order within the capsules is provided through analysis of X-ray scattering data. A novel “Langmuir-Blodgett” mechanism is proposed to account for the formation of the sacs and capsules as the alginate solution is injected at the interface of the PA solution. The capsules show a unique antibacterial effect specific for the Gram positive bacterium Listeria monocytogenes, which is an important human pathogen. The hybrid nanostructured capsules thus have remarkable bioactivity and due to their tunable structural and functional properties are likely to have a diversity of other future applications.

Details

Original languageEnglish
Pages (from-to)8366-8375
Number of pages10
JournalRSC Advances
Volume7
Issue number14
Publication statusPublished - 2017
MoE publication typeA1 Journal article-refereed

Download statistics

No data available

ID: 10958845