Hot electron-induced electrochemiluminescence at cellulose derivatives-based composite electrodes

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@article{bc8bf3f973a44f8487fe3444f764af18,
title = "Hot electron-induced electrochemiluminescence at cellulose derivatives-based composite electrodes",
abstract = "The possibility of using cellulose derivative films as (i) insulating material on metal electrodes or (ii) in composite electrode films on metal to produce hot electron-induced electrochemiluminescence (HECL) was studied. It was shown that the luminophores known to produce HECL at thin insulating film coated cathodes (e.g. Si/SiO2 and Al2O3 electrodes) produced HECL with the present novel electrodes. In the case of composite films consisting of cellulose material doped with conducting carbon particles, the optimal cellulose/carbon black ratios were investigated by measuring the time-resolved HECL (TR-HECL) of an aromatic Tb(III) chelate. In addition to Tb(III) chelate, other well-known labels, fluorescein and Ru(bpy)3 2+ chelate, were demonstrated to produce strong HECL at the present composite electrodes, which are more environmental friendly in disposable assay cartridges as the plastic-based composites we have studied previously. Thus, it is now possible on the present basis to manufacture biodegradable paper-based assay cartridges with HECL detection of labels at biodegradable electrodes. It was shown that the present composite films are stable over wide pH range, and also time-resolved detection of Ru(bpy)3 2+ chelate is possible although its luminescence lifetime if quite short. The calibration curves were measured for presently used aromatic Tb(III) chelate and for Ru(bpy)3 2+. The detection limit (s/n = 3) was 2 · 10−10 M for the Tb(III)-chelate and 4 · 10−9 M for Ru(bpy)3 2+ in time-resolved detection mode. The relative standard deviation for Tb(III)-L1 (n = 5) emission at 10−5 M concentration was 2{\%}. Wide linear range and low detection limits suggests that cellulose based composite electrodes can be used in HECL bioaffinity assays which was finally demonstrated here by an immunometric immunoassay.",
keywords = "Bioaffinity assays, Cellulose derivatives, Electrochemiluminescence, Hot electron electrochemistry, Immunoassays, Lanthanide electrochemiluminescence",
author = "P{\"a}ivi Gr{\"o}nroos and Kalle Salminen and Jouni Paltakari and Qiang Zhang and Nan Wei and Esko Kauppinen and Sakari Kulmala",
year = "2019",
month = "1",
day = "15",
doi = "10.1016/j.jelechem.2018.12.006",
language = "English",
volume = "833",
pages = "349--356",
journal = "Journal of Electroanalytical Chemistry",
issn = "1572-6657",
publisher = "Elsevier Science",

}

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TY - JOUR

T1 - Hot electron-induced electrochemiluminescence at cellulose derivatives-based composite electrodes

AU - Grönroos, Päivi

AU - Salminen, Kalle

AU - Paltakari, Jouni

AU - Zhang, Qiang

AU - Wei, Nan

AU - Kauppinen, Esko

AU - Kulmala, Sakari

PY - 2019/1/15

Y1 - 2019/1/15

N2 - The possibility of using cellulose derivative films as (i) insulating material on metal electrodes or (ii) in composite electrode films on metal to produce hot electron-induced electrochemiluminescence (HECL) was studied. It was shown that the luminophores known to produce HECL at thin insulating film coated cathodes (e.g. Si/SiO2 and Al2O3 electrodes) produced HECL with the present novel electrodes. In the case of composite films consisting of cellulose material doped with conducting carbon particles, the optimal cellulose/carbon black ratios were investigated by measuring the time-resolved HECL (TR-HECL) of an aromatic Tb(III) chelate. In addition to Tb(III) chelate, other well-known labels, fluorescein and Ru(bpy)3 2+ chelate, were demonstrated to produce strong HECL at the present composite electrodes, which are more environmental friendly in disposable assay cartridges as the plastic-based composites we have studied previously. Thus, it is now possible on the present basis to manufacture biodegradable paper-based assay cartridges with HECL detection of labels at biodegradable electrodes. It was shown that the present composite films are stable over wide pH range, and also time-resolved detection of Ru(bpy)3 2+ chelate is possible although its luminescence lifetime if quite short. The calibration curves were measured for presently used aromatic Tb(III) chelate and for Ru(bpy)3 2+. The detection limit (s/n = 3) was 2 · 10−10 M for the Tb(III)-chelate and 4 · 10−9 M for Ru(bpy)3 2+ in time-resolved detection mode. The relative standard deviation for Tb(III)-L1 (n = 5) emission at 10−5 M concentration was 2%. Wide linear range and low detection limits suggests that cellulose based composite electrodes can be used in HECL bioaffinity assays which was finally demonstrated here by an immunometric immunoassay.

AB - The possibility of using cellulose derivative films as (i) insulating material on metal electrodes or (ii) in composite electrode films on metal to produce hot electron-induced electrochemiluminescence (HECL) was studied. It was shown that the luminophores known to produce HECL at thin insulating film coated cathodes (e.g. Si/SiO2 and Al2O3 electrodes) produced HECL with the present novel electrodes. In the case of composite films consisting of cellulose material doped with conducting carbon particles, the optimal cellulose/carbon black ratios were investigated by measuring the time-resolved HECL (TR-HECL) of an aromatic Tb(III) chelate. In addition to Tb(III) chelate, other well-known labels, fluorescein and Ru(bpy)3 2+ chelate, were demonstrated to produce strong HECL at the present composite electrodes, which are more environmental friendly in disposable assay cartridges as the plastic-based composites we have studied previously. Thus, it is now possible on the present basis to manufacture biodegradable paper-based assay cartridges with HECL detection of labels at biodegradable electrodes. It was shown that the present composite films are stable over wide pH range, and also time-resolved detection of Ru(bpy)3 2+ chelate is possible although its luminescence lifetime if quite short. The calibration curves were measured for presently used aromatic Tb(III) chelate and for Ru(bpy)3 2+. The detection limit (s/n = 3) was 2 · 10−10 M for the Tb(III)-chelate and 4 · 10−9 M for Ru(bpy)3 2+ in time-resolved detection mode. The relative standard deviation for Tb(III)-L1 (n = 5) emission at 10−5 M concentration was 2%. Wide linear range and low detection limits suggests that cellulose based composite electrodes can be used in HECL bioaffinity assays which was finally demonstrated here by an immunometric immunoassay.

KW - Bioaffinity assays

KW - Cellulose derivatives

KW - Electrochemiluminescence

KW - Hot electron electrochemistry

KW - Immunoassays

KW - Lanthanide electrochemiluminescence

UR - http://www.scopus.com/inward/record.url?scp=85058394854&partnerID=8YFLogxK

U2 - 10.1016/j.jelechem.2018.12.006

DO - 10.1016/j.jelechem.2018.12.006

M3 - Article

VL - 833

SP - 349

EP - 356

JO - Journal of Electroanalytical Chemistry

JF - Journal of Electroanalytical Chemistry

SN - 1572-6657

ER -

ID: 30782627