Hierarchical assembly of nanostructured coating for siRNA-based dual therapy of bone regeneration and revascularization

Research output: Contribution to journalArticleScientificpeer-review

Researchers

  • Helin Xing
  • Xing Wang
  • Gao Xiao
  • Zongmin Zhao
  • Shiquan Zou
  • Man Li
  • Joseph J. Richardson
  • Blaise L. Tardy
  • Liangxia Xie
  • Satoshi Komasa
  • Joji Okazaki
  • Qingsong Jiang
  • Guodong Yang
  • Junling Guo

Research units

  • Capital Medical University
  • Shanxi Medical University
  • Sichuan University
  • Harvard University
  • University of Melbourne
  • Osaka Dental University
  • Fourth Military Medical University

Abstract

Advancing bone implant engineering offers the opportunity to overcome crucial medical challenges and improve clinical outcomes. Although the establishment of a functional vascular network is crucial for bone development, its regeneration inside bone tissue has only received limited attention to date. Herein, we utilize siRNA-decorated particles to engineer a hierarchical nanostructured coating on clinically used titanium implants for the synergistic regeneration of skeletal and vascular tissues. Specifically, an siRNA was designed to target the regulation of cathepsin K and conjugated on nanoparticles. The functionalized nanoparticles were assembled onto the bone implant to form a hierarchical nanostructured coating. By regulating mRNA transcription, the coating significantly promotes cell viability and growth factor release related to vascularization. Moreover, microchip-based experiments demonstrate that the nanostructured coating facilitates macrophage-induced synergy in up-regulation of at least seven bone and vascular growth factors. Ovariectomized rat and comprehensive beagle dog models highlight that this siRNA-integrated nanostructured coating possesses all the key traits of a clinically promising candidate to address the myriad of challenges associated with bone regeneration.

Details

Original languageEnglish
Article number119784
JournalBiomaterials
Volume235
Publication statusPublished - 1 Mar 2020
MoE publication typeA1 Journal article-refereed

    Research areas

  • Bone regeneration, Colloidal assembly, Revascularization, siRNA, Surface functionalization

ID: 40827429