Hemicellulose–Cellulose Composites Reveal Differences in Cellulose Organization after Dilute Acid Pretreatment

Research output: Contribution to journalArticleScientificpeer-review

Researchers

  • Riddhi Shah
  • Shixin Huang
  • Sai Venkatesh Pingali
  • Daisuke Sawada

  • Yunqiao Pu
  • Miguel Rodriguez
  • A Ragauskas
  • Seong H. Kim
  • Evans Barbara
  • Brian Davison
  • Hugh M. O'Neill

Research units

  • Oak Ridge National Laboratory
  • Pennsylvania State University

Abstract

Model hemicellulose-cellulose composites that mimic plant cell wall polymer interactions were prepared by synthesizing deuterated bacterial cellulose in the presence of glucomannan or xyloglucan. Dilute acid pretreatment (DAP) of these materials was studied using small-angle neutron scattering, X-ray diffraction, and sum frequency generation spectroscopy. The macrofibril dimensions of the pretreated cellulose alone were smaller but with similar entanglement of macrofibrillar network as native cellulose. In addition, the crystallite size dimension along the (010) plane increased. Glucomannan-cellulose underwent similar changes to cellulose, except that the macrofibrillar network was more entangled after DAP. Conversely, in xyloglucan-cellulose the macrofibril dimensions and macrofibrillar network were relatively unchanged after pretreatment, but the cellulose I-beta content was increased. Our results point to a tight interaction of xyloglucan with microfibrils while glucomannan only interacts with macrofibril surfaces. This study provides insight into roles of different hemicellulose-cellulose interactions and may help in improving pretreatment processes or engineering plants with decreased recalcitrance.

Details

Original languageEnglish
Pages (from-to)893-903
Number of pages11
JournalBiomacromolecules
Volume20
Issue number2
Publication statusPublished - 11 Feb 2019
MoE publication typeA1 Journal article-refereed

    Research areas

  • ACETOBACTER-XYLINUM, BACTERIAL CELLULOSE, BIOSYNTHESIS, CRYSTAL-STRUCTURE, HYDROLYSIS, PATTERNS, WALL MATRIX POLYSACCHARIDES, WHEAT-STRAW, X-RAY, XYLOGLUCAN

ID: 31361724