Graphene-Augmented Nanofiber Scaffolds Trigger Gene Expressions Switching of Four Cancer Cell Types

Jekaterina Kazantseva, Roman Ivanov, Michael M. Gasik, Toomas Neuman, Irina Hussainova

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)
167 Downloads (Pure)


Three-dimensional (3D) customized scaffolds are anticipated in providing new frontiers in cells manipulation and advanced therapy methods. Here we demonstrate the application of hybrid 3D porous scaffolds, representing networks of highly aligned self-assembled ceramic nano-fibers, for culturing four types of cancer cells. Ultra-high aspect ratio (~~107) of graphene augmented fibers of tailored nano-topology is shown as an alternative tool to substantially affect cancerous gene expression, eventually due to differences in local biomechanical features of the cell-matrix interactions. Here we report a clear selective up- and down-regulation of groups of markers for breast cancer (MDA-MB231), colorectal cancer (CaCO2), melanoma (WM239A) and neuroblastoma (Kelly) depending on only fibers orientation and morphology without application of any other stimulus. Changes in gene expression are also revealed for Mitomycin C treatment of MDA-MB231, making the scaffold a suitable platform for testing of anticancer agents. This allows an opportunity for selective »clean» guidance to deep understanding mechanisms of cancer cells progressive growth and tumor formation without possible side effects by manipulation with the specific markers.

Original languageEnglish
Pages (from-to)1622–1629
Number of pages8
JournalACS Biomaterials Science and Engineering
Issue number5
Publication statusPublished - Apr 2018
MoE publication typeA1 Journal article-refereed


Dive into the research topics of 'Graphene-Augmented Nanofiber Scaffolds Trigger Gene Expressions Switching of Four Cancer Cell Types'. Together they form a unique fingerprint.

Cite this