Glucose oxidase - An overview

Sandip B. Bankar, Mahesh V. Bule, Rekha S. Singhal, Laxmi Ananthanarayan*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

722 Citations (Scopus)


Glucose oxidase (β-d-glucose:oxygen 1-oxidoreductase; EC catalyzes the oxidation of β-d-glucose to gluconic acid, by utilizing molecular oxygen as an electron acceptor with simultaneous production of hydrogen peroxide. Microbial glucose oxidase is currently receiving much attention due to its wide applications in chemical, pharmaceutical, food, beverage, clinical chemistry, biotechnology and other industries. Novel applications of glucose oxidase in biosensors have increased the demand in recent years. Present review discusses the production, recovery, characterization, immobilization and applications of glucose oxidase. Production of glucose oxidase by fermentation is detailed, along with recombinant methods. Various purification techniques for higher recovery of glucose oxidase are described here. Issues of enzyme kinetics, stability studies and characterization are addressed. Immobilized preparations of glucose oxidase are also discussed. Applications of glucose oxidase in various industries and as analytical enzymes are having an increasing impact on bioprocessing.

Original languageEnglish
Pages (from-to)489-501
Number of pages13
JournalBiotechnology Advances
Issue number4
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed


  • Application in biosensors
  • Catalse
  • Downstream processing
  • Glucose oxidase
  • Immobilization
  • Optimization of fermentation parameters


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