Genetic engineering of S. cerevisiae for pentose utilization

Peter Richard; Ritva Verho; John Londesborough; Merja Penttilä

doi:10.1021/bk-2006-0921.ch014

Genetic engineering of S. cerevisiae for pentose utilization

Peter Richard^*
, Ritva Verho
, John Londesborough
, Merja Penttilä

^*Corresponding author for this work

Not published at Aalto University

VTT Technical Research Centre of Finland

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

1 Citation (Web of Science)

Abstract

Recombinant Saccharomyces cerevisiae able to ferment the pentoses D-xylose and L-arabinose, was modified for improved fermentation rates and yields Pentose fermentation is relevant when low cost raw materials such as plant hydrolysates are fermented to ethanol The two most widespread pentose sugars in our biosphere are D-xylose and L-arabinose S cerevisiae is unable to ferment pentoses but has been engineered to do so, however rates and yields are low The imbalance of redox cofactors (excess NADP and NADH are produced) is considered a major limiting factor For the L-arabinose fermentation we identified an NADH-dependent L-xylulose reductase replacing the previously known NADPH-dependent enzyme For D-xylose fermentation we introduced an NADP-dependent glyceraldehyde 3-phospate dehydrogenase to regenerate NADPH.

Original language	English
Title of host publication	Feedstocks for the Future
Subtitle of host publication	Renewables for the Production of Chemicals and Materials
Editors	JJ Bozell, MK Patel
Publisher	American Chemical Society
Chapter	14
Pages	184-192
Number of pages	9
ISBN (Print)	0-8412-3934-7
DOIs	https://doi.org/10.1021/bk-2006-0921.ch014
Publication status	Published - 2006
MoE publication type	A4 Conference publication
Event	American Chemical Society National Meeting & Exposition - Anaheim, United States Duration: 28 Mar 2004 → 1 Apr 2004 Conference number: 227

Publication series

Name	ACS SYMPOSIUM SERIES
Publisher	American Chemical Society
Volume	921
ISSN (Print)	0097-6156

Conference

Conference	American Chemical Society National Meeting & Exposition
Abbreviated title	ACS
Country/Territory	United States
City	Anaheim
Period	28/03/2004 → 01/04/2004

Keywords

ARABINOSE CATABOLIC PATHWAY
YEAST PICHIA-STIPITIS
L-XYLULOSE REDUCTASE
XYLOSE FERMENTATION
XYLITOL-DEHYDROGENASE
FUNCTIONAL EXPRESSION
FERMENTING YEASTS
ESCHERICHIA-COLI
ETHANOL
XYLULOKINASE

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10.1021/bk-2006-0921.ch014

Cite this

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title = "Genetic engineering of S. cerevisiae for pentose utilization",

abstract = "Recombinant Saccharomyces cerevisiae able to ferment the pentoses D-xylose and L-arabinose, was modified for improved fermentation rates and yields Pentose fermentation is relevant when low cost raw materials such as plant hydrolysates are fermented to ethanol The two most widespread pentose sugars in our biosphere are D-xylose and L-arabinose S cerevisiae is unable to ferment pentoses but has been engineered to do so, however rates and yields are low The imbalance of redox cofactors (excess NADP and NADH are produced) is considered a major limiting factor For the L-arabinose fermentation we identified an NADH-dependent L-xylulose reductase replacing the previously known NADPH-dependent enzyme For D-xylose fermentation we introduced an NADP-dependent glyceraldehyde 3-phospate dehydrogenase to regenerate NADPH.",

keywords = "ARABINOSE CATABOLIC PATHWAY, YEAST PICHIA-STIPITIS, L-XYLULOSE REDUCTASE, XYLOSE FERMENTATION, XYLITOL-DEHYDROGENASE, FUNCTIONAL EXPRESSION, FERMENTING YEASTS, ESCHERICHIA-COLI, ETHANOL, XYLULOKINASE",

author = "Peter Richard and Ritva Verho and John Londesborough and Merja Penttil{\"a}",

year = "2006",

doi = "10.1021/bk-2006-0921.ch014",

language = "English",

isbn = "0-8412-3934-7",

series = "ACS SYMPOSIUM SERIES",

publisher = "American Chemical Society",

pages = "184--192",

editor = "JJ Bozell and MK Patel",

booktitle = "Feedstocks for the Future",

address = "United States",

note = "American Chemical Society National Meeting \& Exposition, ACS ; Conference date: 28-03-2004 Through 01-04-2004",

}

Richard, P, Verho, R, Londesborough, J & Penttilä, M 2006, Genetic engineering of S. cerevisiae for pentose utilization. in JJ Bozell & MK Patel (eds), Feedstocks for the Future: Renewables for the Production of Chemicals and Materials. ACS SYMPOSIUM SERIES, vol. 921, American Chemical Society, pp. 184-192, American Chemical Society National Meeting & Exposition, Anaheim, California, United States, 28/03/2004. https://doi.org/10.1021/bk-2006-0921.ch014

Genetic engineering of S. cerevisiae for pentose utilization. / Richard, Peter; Verho, Ritva; Londesborough, John et al.
Feedstocks for the Future: Renewables for the Production of Chemicals and Materials. ed. / JJ Bozell; MK Patel. American Chemical Society, 2006. p. 184-192 (ACS SYMPOSIUM SERIES; Vol. 921).

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

TY - GEN

T1 - Genetic engineering of S. cerevisiae for pentose utilization

AU - Richard, Peter

AU - Verho, Ritva

AU - Londesborough, John

AU - Penttilä, Merja

N1 - Conference code: 227

PY - 2006

Y1 - 2006

N2 - Recombinant Saccharomyces cerevisiae able to ferment the pentoses D-xylose and L-arabinose, was modified for improved fermentation rates and yields Pentose fermentation is relevant when low cost raw materials such as plant hydrolysates are fermented to ethanol The two most widespread pentose sugars in our biosphere are D-xylose and L-arabinose S cerevisiae is unable to ferment pentoses but has been engineered to do so, however rates and yields are low The imbalance of redox cofactors (excess NADP and NADH are produced) is considered a major limiting factor For the L-arabinose fermentation we identified an NADH-dependent L-xylulose reductase replacing the previously known NADPH-dependent enzyme For D-xylose fermentation we introduced an NADP-dependent glyceraldehyde 3-phospate dehydrogenase to regenerate NADPH.

AB - Recombinant Saccharomyces cerevisiae able to ferment the pentoses D-xylose and L-arabinose, was modified for improved fermentation rates and yields Pentose fermentation is relevant when low cost raw materials such as plant hydrolysates are fermented to ethanol The two most widespread pentose sugars in our biosphere are D-xylose and L-arabinose S cerevisiae is unable to ferment pentoses but has been engineered to do so, however rates and yields are low The imbalance of redox cofactors (excess NADP and NADH are produced) is considered a major limiting factor For the L-arabinose fermentation we identified an NADH-dependent L-xylulose reductase replacing the previously known NADPH-dependent enzyme For D-xylose fermentation we introduced an NADP-dependent glyceraldehyde 3-phospate dehydrogenase to regenerate NADPH.

KW - ARABINOSE CATABOLIC PATHWAY

KW - YEAST PICHIA-STIPITIS

KW - L-XYLULOSE REDUCTASE

KW - XYLOSE FERMENTATION

KW - XYLITOL-DEHYDROGENASE

KW - FUNCTIONAL EXPRESSION

KW - FERMENTING YEASTS

KW - ESCHERICHIA-COLI

KW - ETHANOL

KW - XYLULOKINASE

U2 - 10.1021/bk-2006-0921.ch014

DO - 10.1021/bk-2006-0921.ch014

M3 - Conference article in proceedings

SN - 0-8412-3934-7

T3 - ACS SYMPOSIUM SERIES

SP - 184

EP - 192

BT - Feedstocks for the Future

A2 - Bozell, JJ

A2 - Patel, MK

PB - American Chemical Society

T2 - American Chemical Society National Meeting & Exposition

Y2 - 28 March 2004 through 1 April 2004

ER -

Genetic engineering of S. cerevisiae for pentose utilization

Abstract

Publication series

Conference

Keywords

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