GENERIC METHOD FOR ATTACHING BIOMOLECULES VIA AVIDIN-BIOTIN COMPLEXES IMMOBILIZED ON FILMS OF REGENERATED CELLULOSE

H. Orelma; L. -S. Johansson; Ilari Filpponen; Orlando J. Rojas; Janne Laine

GENERIC METHOD FOR ATTACHING BIOMOLECULES VIA AVIDIN-BIOTIN COMPLEXES IMMOBILIZED ON FILMS OF REGENERATED CELLULOSE

H. Orelma
, L. -S. Johansson
, Ilari Filpponen^*
, Orlando J. Rojas
, Janne Laine

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

Abstract

We investigated the adsorption and chemical conjugation of avidin and its deglycosylated form, neutravidin, on films of regenerated cellulose. The dynamics and extent of biomolecular attachment were monitored in-situ by quartz crystal microbalance microgravimetry and ex-situ via X-ray photoelectron spectroscopy. The installation of carboxyl groups on cellulose after modification with carboxymethylated cellulose (CMC) significantly increased physisorption of avidins, which can be then covalently conjugated by using EDS/NHS coupling chemistries. The developed cellulose-avidin biointerfaces are able to scavenge biotinylated molecules from solution as demonstrated by successful surface complexation of biotinylated bovine serum albumin (Biotin-BSA).

Original language	English
Title of host publication	PROCEEDING OF THE 4TH INTERNATIONAL CONFERENCE ON PULPING, PAPERMAKING AND BIOTECHNOLOGY (ICPPB '12), VOLS. I AND II
Editors	Y Jin, Z Wang, W Wu
Publisher	Nanjing Forestry University
Pages	896-901
Number of pages	6
ISBN (Print)	978-7-5019-9043-6
Publication status	Published - 2012
MoE publication type	A4 Conference publication
Event	International Conference on Pulping, Papermaking and Biotechnology - Nanjing, China Duration: 7 Nov 2012 → 9 Nov 2012 Conference number: 4

Conference

Conference	International Conference on Pulping, Papermaking and Biotechnology
Abbreviated title	ICPPB
Country/Territory	China
City	Nanjing
Period	07/11/2012 → 09/11/2012

Keywords

avidin
biotin
cellulose
carboxymethyl cellulose
QCM-D
NUCLEIC-ACID
ATTACHMENT
SUBUNIT
PEPTIDE
CHAIN

Cite this

Orelma, H., Johansson, L. .-S., Filpponen, I., Rojas, O. J., & Laine, J. (2012). GENERIC METHOD FOR ATTACHING BIOMOLECULES VIA AVIDIN-BIOTIN COMPLEXES IMMOBILIZED ON FILMS OF REGENERATED CELLULOSE. In Y. Jin, Z. Wang, & W. Wu (Eds.), PROCEEDING OF THE 4TH INTERNATIONAL CONFERENCE ON PULPING, PAPERMAKING AND BIOTECHNOLOGY (ICPPB '12), VOLS. I AND II (pp. 896-901). Nanjing Forestry University.

Orelma, H. ; Johansson, L. -S. ; Filpponen, Ilari et al. / GENERIC METHOD FOR ATTACHING BIOMOLECULES VIA AVIDIN-BIOTIN COMPLEXES IMMOBILIZED ON FILMS OF REGENERATED CELLULOSE. PROCEEDING OF THE 4TH INTERNATIONAL CONFERENCE ON PULPING, PAPERMAKING AND BIOTECHNOLOGY (ICPPB '12), VOLS. I AND II. editor / Y Jin ; Z Wang ; W Wu. Nanjing Forestry University, 2012. pp. 896-901

@inproceedings{2a4f81324d4c4216bf08dd18315f7c67,

title = "GENERIC METHOD FOR ATTACHING BIOMOLECULES VIA AVIDIN-BIOTIN COMPLEXES IMMOBILIZED ON FILMS OF REGENERATED CELLULOSE",

abstract = "We investigated the adsorption and chemical conjugation of avidin and its deglycosylated form, neutravidin, on films of regenerated cellulose. The dynamics and extent of biomolecular attachment were monitored in-situ by quartz crystal microbalance microgravimetry and ex-situ via X-ray photoelectron spectroscopy. The installation of carboxyl groups on cellulose after modification with carboxymethylated cellulose (CMC) significantly increased physisorption of avidins, which can be then covalently conjugated by using EDS/NHS coupling chemistries. The developed cellulose-avidin biointerfaces are able to scavenge biotinylated molecules from solution as demonstrated by successful surface complexation of biotinylated bovine serum albumin (Biotin-BSA).",

keywords = "avidin, biotin, cellulose, carboxymethyl cellulose, QCM-D, NUCLEIC-ACID, ATTACHMENT, SUBUNIT, PEPTIDE, CHAIN",

author = "H. Orelma and Johansson, \{L. -S.\} and Ilari Filpponen and Rojas, \{Orlando J.\} and Janne Laine",

year = "2012",

language = "English",

isbn = "978-7-5019-9043-6",

pages = "896--901",

editor = "Y Jin and Z Wang and W Wu",

booktitle = "PROCEEDING OF THE 4TH INTERNATIONAL CONFERENCE ON PULPING, PAPERMAKING AND BIOTECHNOLOGY (ICPPB '12), VOLS. I AND II",

publisher = "Nanjing Forestry University",

address = "China",

note = "International Conference on Pulping, Papermaking and Biotechnology, ICPPB ; Conference date: 07-11-2012 Through 09-11-2012",

}

Orelma, H, Johansson, L-S, Filpponen, I, Rojas, OJ & Laine, J 2012, GENERIC METHOD FOR ATTACHING BIOMOLECULES VIA AVIDIN-BIOTIN COMPLEXES IMMOBILIZED ON FILMS OF REGENERATED CELLULOSE. in Y Jin, Z Wang & W Wu (eds), PROCEEDING OF THE 4TH INTERNATIONAL CONFERENCE ON PULPING, PAPERMAKING AND BIOTECHNOLOGY (ICPPB '12), VOLS. I AND II. Nanjing Forestry University, pp. 896-901, International Conference on Pulping, Papermaking and Biotechnology, Nanjing, China, 07/11/2012.

GENERIC METHOD FOR ATTACHING BIOMOLECULES VIA AVIDIN-BIOTIN COMPLEXES IMMOBILIZED ON FILMS OF REGENERATED CELLULOSE. / Orelma, H.; Johansson, L. -S.; Filpponen, Ilari et al.
PROCEEDING OF THE 4TH INTERNATIONAL CONFERENCE ON PULPING, PAPERMAKING AND BIOTECHNOLOGY (ICPPB '12), VOLS. I AND II. ed. / Y Jin; Z Wang; W Wu. Nanjing Forestry University, 2012. p. 896-901.

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

TY - GEN

T1 - GENERIC METHOD FOR ATTACHING BIOMOLECULES VIA AVIDIN-BIOTIN COMPLEXES IMMOBILIZED ON FILMS OF REGENERATED CELLULOSE

AU - Orelma, H.

AU - Johansson, L. -S.

AU - Filpponen, Ilari

AU - Rojas, Orlando J.

AU - Laine, Janne

N1 - Conference code: 4

PY - 2012

Y1 - 2012

N2 - We investigated the adsorption and chemical conjugation of avidin and its deglycosylated form, neutravidin, on films of regenerated cellulose. The dynamics and extent of biomolecular attachment were monitored in-situ by quartz crystal microbalance microgravimetry and ex-situ via X-ray photoelectron spectroscopy. The installation of carboxyl groups on cellulose after modification with carboxymethylated cellulose (CMC) significantly increased physisorption of avidins, which can be then covalently conjugated by using EDS/NHS coupling chemistries. The developed cellulose-avidin biointerfaces are able to scavenge biotinylated molecules from solution as demonstrated by successful surface complexation of biotinylated bovine serum albumin (Biotin-BSA).

AB - We investigated the adsorption and chemical conjugation of avidin and its deglycosylated form, neutravidin, on films of regenerated cellulose. The dynamics and extent of biomolecular attachment were monitored in-situ by quartz crystal microbalance microgravimetry and ex-situ via X-ray photoelectron spectroscopy. The installation of carboxyl groups on cellulose after modification with carboxymethylated cellulose (CMC) significantly increased physisorption of avidins, which can be then covalently conjugated by using EDS/NHS coupling chemistries. The developed cellulose-avidin biointerfaces are able to scavenge biotinylated molecules from solution as demonstrated by successful surface complexation of biotinylated bovine serum albumin (Biotin-BSA).

KW - avidin

KW - biotin

KW - cellulose

KW - carboxymethyl cellulose

KW - QCM-D

KW - NUCLEIC-ACID

KW - ATTACHMENT

KW - SUBUNIT

KW - PEPTIDE

KW - CHAIN

M3 - Conference article in proceedings

SN - 978-7-5019-9043-6

SP - 896

EP - 901

BT - PROCEEDING OF THE 4TH INTERNATIONAL CONFERENCE ON PULPING, PAPERMAKING AND BIOTECHNOLOGY (ICPPB '12), VOLS. I AND II

A2 - Jin, Y

A2 - Wang, Z

A2 - Wu, W

PB - Nanjing Forestry University

T2 - International Conference on Pulping, Papermaking and Biotechnology

Y2 - 7 November 2012 through 9 November 2012

ER -

GENERIC METHOD FOR ATTACHING BIOMOLECULES VIA AVIDIN-BIOTIN COMPLEXES IMMOBILIZED ON FILMS OF REGENERATED CELLULOSE

Abstract

Conference

Keywords

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Cite this