Abstract
We investigated the adsorption and chemical conjugation of avidin and its deglycosylated form, neutravidin, on films of regenerated cellulose. The dynamics and extent of biomolecular attachment were monitored in-situ by quartz crystal microbalance microgravimetry and ex-situ via X-ray photoelectron spectroscopy. The installation of carboxyl groups on cellulose after modification with carboxymethylated cellulose (CMC) significantly increased physisorption of avidins, which can be then covalently conjugated by using EDS/NHS coupling chemistries. The developed cellulose-avidin biointerfaces are able to scavenge biotinylated molecules from solution as demonstrated by successful surface complexation of biotinylated bovine serum albumin (Biotin-BSA).
Original language | English |
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Title of host publication | PROCEEDING OF THE 4TH INTERNATIONAL CONFERENCE ON PULPING, PAPERMAKING AND BIOTECHNOLOGY (ICPPB '12), VOLS. I AND II |
Editors | Y Jin, Z Wang, W Wu |
Pages | 896-901 |
Number of pages | 6 |
Publication status | Published - 2012 |
MoE publication type | A4 Article in a conference publication |
Event | International Conference on Pulping, Papermaking and Biotechnology - Nanjing, China Duration: 7 Nov 2012 → 9 Nov 2012 Conference number: 4 |
Conference
Conference | International Conference on Pulping, Papermaking and Biotechnology |
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Abbreviated title | ICPPB |
Country/Territory | China |
City | Nanjing |
Period | 07/11/2012 → 09/11/2012 |
Keywords
- avidin
- biotin
- cellulose
- carboxymethyl cellulose
- QCM-D
- NUCLEIC-ACID
- ATTACHMENT
- SUBUNIT
- PEPTIDE
- CHAIN