Abstract
We investigated the adsorption and chemical conjugation of avidin and its deglycosylated form, neutravidin, on films of regenerated cellulose. The dynamics and extent of biomolecular attachment were monitored in-situ by quartz crystal microbalance microgravimetry and ex-situ via X-ray photoelectron spectroscopy. The installation of carboxyl groups on cellulose after modification with carboxymethylated cellulose (CMC) significantly increased physisorption of avidins, which can be then covalently conjugated by using EDS/NHS coupling chemistries. The developed cellulose-avidin biointerfaces are able to scavenge biotinylated molecules from solution as demonstrated by successful surface complexation of biotinylated bovine serum albumin (Biotin-BSA).
| Original language | English |
|---|---|
| Title of host publication | PROCEEDING OF THE 4TH INTERNATIONAL CONFERENCE ON PULPING, PAPERMAKING AND BIOTECHNOLOGY (ICPPB '12), VOLS. I AND II |
| Editors | Y Jin, Z Wang, W Wu |
| Pages | 896-901 |
| Number of pages | 6 |
| Publication status | Published - 2012 |
| MoE publication type | A4 Article in a conference publication |
| Event | International Conference on Pulping, Papermaking and Biotechnology - Nanjing, China Duration: 7 Nov 2012 → 9 Nov 2012 Conference number: 4 |
Conference
| Conference | International Conference on Pulping, Papermaking and Biotechnology |
|---|---|
| Abbreviated title | ICPPB |
| Country/Territory | China |
| City | Nanjing |
| Period | 07/11/2012 → 09/11/2012 |
Keywords
- avidin
- biotin
- cellulose
- carboxymethyl cellulose
- QCM-D
- NUCLEIC-ACID
- ATTACHMENT
- SUBUNIT
- PEPTIDE
- CHAIN