GENERIC METHOD FOR ATTACHING BIOMOLECULES VIA AVIDIN-BIOTIN COMPLEXES IMMOBILIZED ON FILMS OF REGENERATED CELLULOSE

H. Orelma, L. -S. Johansson, Ilari Filpponen*, Orlando J. Rojas, Janne Laine

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

    Abstract

    We investigated the adsorption and chemical conjugation of avidin and its deglycosylated form, neutravidin, on films of regenerated cellulose. The dynamics and extent of biomolecular attachment were monitored in-situ by quartz crystal microbalance microgravimetry and ex-situ via X-ray photoelectron spectroscopy. The installation of carboxyl groups on cellulose after modification with carboxymethylated cellulose (CMC) significantly increased physisorption of avidins, which can be then covalently conjugated by using EDS/NHS coupling chemistries. The developed cellulose-avidin biointerfaces are able to scavenge biotinylated molecules from solution as demonstrated by successful surface complexation of biotinylated bovine serum albumin (Biotin-BSA).

    Original languageEnglish
    Title of host publicationPROCEEDING OF THE 4TH INTERNATIONAL CONFERENCE ON PULPING, PAPERMAKING AND BIOTECHNOLOGY (ICPPB '12), VOLS. I AND II
    EditorsY Jin, Z Wang, W Wu
    Pages896-901
    Number of pages6
    Publication statusPublished - 2012
    MoE publication typeA4 Article in a conference publication
    EventInternational Conference on Pulping, Papermaking and Biotechnology - Nanjing, China
    Duration: 7 Nov 20129 Nov 2012
    Conference number: 4

    Conference

    ConferenceInternational Conference on Pulping, Papermaking and Biotechnology
    Abbreviated titleICPPB
    CountryChina
    CityNanjing
    Period07/11/201209/11/2012

    Keywords

    • avidin
    • biotin
    • cellulose
    • carboxymethyl cellulose
    • QCM-D
    • NUCLEIC-ACID
    • ATTACHMENT
    • SUBUNIT
    • PEPTIDE
    • CHAIN

    Cite this