Generation of Functional Coatings on Hydrophobic Surfaces through Deposition of Denatured Proteins Followed by Grafting from Polymerization

Kiran K. Goli, Orlando J. Rojas, A. Evren Oezcam, Jan Genzer*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

26 Citations (Scopus)

Abstract

Hydrophilic coatings were produced on flat hydrophobic substrates featuring n-octadecyltrichlorosilane (ODTS) and synthetic polypropylene (PP) nonwoven surfaces through the adsorption of denatured proteins. Specifically, physisorption from aqueous solutions of alpha-lactalbumin, lysozyme, fibrinogen, and two soy globulin proteins (glycinin and beta-conglycinin) after chemical (urea) and thermal denaturation endowed the hydrophobic surfaces with amino and hydroxyl functionalities, yielding enhanced wettability. Proteins adsorbed strongly onto ODTS and PP through nonspecific interactions. The thickness of adsorbed heat-denatured proteins was adjusted by varying the pH, protein concentration in solution, and adsorption time. In addition, the stability of the immobilized protein layer was improved significantly after interfacial cross-linking with glutaraldehyde in the presence of sodium borohydride. The amino and hydroxyl groups present on the protein-modified surfaces served as reactive sites for the attachment of polymerization initiators from which polymer brushes were grown by surface-initiated atom-transfer radical polymerization of 2-hydroxyethyl methacrylate. Protein denaturation and adsorption as well as the grafting of polymeric brushes were characterized by circular dichroism, ellipsometry, contact angle, and Fourier transform infrared spectroscopy in the attenuated total reflection mode.

Original languageEnglish
Pages (from-to)1371-1382
Number of pages12
JournalBiomacromolecules
Volume13
Issue number5
DOIs
Publication statusPublished - May 2012
MoE publication typeA1 Journal article-refereed

Keywords

  • TRANSFER RADICAL POLYMERIZATION
  • POLYPROPYLENE MICROPOROUS MEMBRANES
  • DIFFERENTIAL SCANNING CALORIMETRY
  • SELF-ASSEMBLED MONOLAYERS
  • SOLID-SURFACES
  • CONFORMATIONAL-CHANGES
  • CIRCULAR-DICHROISM
  • HUMAN-FIBRINOGEN
  • CROSS-LINKING
  • THERMAL-DENATURATION

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