Fungal loosenin-like proteins boost the cellulolytic enzyme conversion of pretreated wood fiber and cellulosic pulps

Deepika Dahiya; Taru Koitto; Kim Kutvonen; Yan Wang; Majid Haddad Momeni; Siiri de Ruijter; Emma R. Master

doi:10.1016/j.biortech.2023.130188

Fungal loosenin-like proteins boost the cellulolytic enzyme conversion of pretreated wood fiber and cellulosic pulps

Deepika Dahiya, Taru Koitto, Kim Kutvonen, Yan Wang, Majid Haddad Momeni, Siiri de Ruijter, Emma R. Master^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

Microbial expansin-related proteins, including loosenins, can disrupt cellulose networks and increase enzyme accessibility to cellulosic substrates. Herein, four loosenins from Phanerochaete carnosa (PcaLOOLs), and a PcaLOOL fused to a family 63 carbohydrate-binding module, were compared for ability to boost the cellulolytic deconstruction of steam pretreated softwood (SSW) and kraft pulps from softwood (ND-BSKP) and hardwood (ND-BHKP). Amending the Cellic® CTec-2 cellulase cocktail with PcaLOOLs increased reducing products from SSW by up to 40 %, corresponding to 28 % higher glucose yield. Amending Cellic® CTec-2 with PcaLOOLs also increased the release of glucose from ND-BSKP and ND-BHKP by 82 % and 28 %, respectively. Xylose release from ND-BSKP and ND-BHKP increased by 47 % and 57 %, respectively, highlighting the potential of PcaLOOLs to enhance hemicellulose recovery. Scanning electron microscopy and fiber image analysis revealed fibrillation and curlation of ND-BSKP after PcaLOOL treatment, consistent with increasing enzyme accessibility to targeted substrates.

Original language	English
Article number	130188
Number of pages	8
Journal	Bioresource Technology
Volume	394
Early online date	21 Dec 2023
DOIs	https://doi.org/10.1016/j.biortech.2023.130188
Publication status	Published - Feb 2024
MoE publication type	A1 Journal article-refereed

Keywords

Bioprocessing
Carbohydrate-active enzymes
Lignocellulose
Loosenins
Microbial expansins

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2023.130188Licence: CC BY

CHEM_Dahiya_et_al_Fungal_loosenin-like_2024_Bioresource_TechnologyFinal published version, 2.01 MBLicence: CC BY

Cite this

@article{3f40d08f26ef4e4895b118e016a2ce91,

title = "Fungal loosenin-like proteins boost the cellulolytic enzyme conversion of pretreated wood fiber and cellulosic pulps",

abstract = "Microbial expansin-related proteins, including loosenins, can disrupt cellulose networks and increase enzyme accessibility to cellulosic substrates. Herein, four loosenins from Phanerochaete carnosa (PcaLOOLs), and a PcaLOOL fused to a family 63 carbohydrate-binding module, were compared for ability to boost the cellulolytic deconstruction of steam pretreated softwood (SSW) and kraft pulps from softwood (ND-BSKP) and hardwood (ND-BHKP). Amending the Cellic{\textregistered} CTec-2 cellulase cocktail with PcaLOOLs increased reducing products from SSW by up to 40 %, corresponding to 28 % higher glucose yield. Amending Cellic{\textregistered} CTec-2 with PcaLOOLs also increased the release of glucose from ND-BSKP and ND-BHKP by 82 % and 28 %, respectively. Xylose release from ND-BSKP and ND-BHKP increased by 47 % and 57 %, respectively, highlighting the potential of PcaLOOLs to enhance hemicellulose recovery. Scanning electron microscopy and fiber image analysis revealed fibrillation and curlation of ND-BSKP after PcaLOOL treatment, consistent with increasing enzyme accessibility to targeted substrates.",

keywords = "Bioprocessing, Carbohydrate-active enzymes, Lignocellulose, Loosenins, Microbial expansins",

author = "Deepika Dahiya and Taru Koitto and Kim Kutvonen and Yan Wang and {Haddad Momeni}, Majid and {de Ruijter}, Siiri and Master, {Emma R.}",

note = "| openaire: EC/H2020/964764/EU//BioUPGRADE Funding Information: This project received funding from the European Union{\textquoteright}s Horizon 2020 research and innovation programme under grant agreement No 964764 and Business Finland under grant agreement 7655/31/2019. The content presented in this document represents the views of the authors and the Commission is not responsible for any use that may be made of the information it contains. T.K. was supported by the Jenny and Antti Wihuri Foundation (Centre for Young Synbio Scientists). This work has made use of Aalto University{\textquoteright}s Bioeconomy facilities. Publisher Copyright: {\textcopyright} 2023 The Author(s)",

year = "2024",

month = feb,

doi = "10.1016/j.biortech.2023.130188",

language = "English",

volume = "394",

journal = "Bioresource Technology",

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T1 - Fungal loosenin-like proteins boost the cellulolytic enzyme conversion of pretreated wood fiber and cellulosic pulps

AU - Dahiya, Deepika

AU - Koitto, Taru

AU - Kutvonen, Kim

AU - Wang, Yan

AU - Haddad Momeni, Majid

AU - de Ruijter, Siiri

AU - Master, Emma R.

N1 - | openaire: EC/H2020/964764/EU//BioUPGRADE Funding Information: This project received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 964764 and Business Finland under grant agreement 7655/31/2019. The content presented in this document represents the views of the authors and the Commission is not responsible for any use that may be made of the information it contains. T.K. was supported by the Jenny and Antti Wihuri Foundation (Centre for Young Synbio Scientists). This work has made use of Aalto University’s Bioeconomy facilities. Publisher Copyright: © 2023 The Author(s)

PY - 2024/2

Y1 - 2024/2

N2 - Microbial expansin-related proteins, including loosenins, can disrupt cellulose networks and increase enzyme accessibility to cellulosic substrates. Herein, four loosenins from Phanerochaete carnosa (PcaLOOLs), and a PcaLOOL fused to a family 63 carbohydrate-binding module, were compared for ability to boost the cellulolytic deconstruction of steam pretreated softwood (SSW) and kraft pulps from softwood (ND-BSKP) and hardwood (ND-BHKP). Amending the Cellic® CTec-2 cellulase cocktail with PcaLOOLs increased reducing products from SSW by up to 40 %, corresponding to 28 % higher glucose yield. Amending Cellic® CTec-2 with PcaLOOLs also increased the release of glucose from ND-BSKP and ND-BHKP by 82 % and 28 %, respectively. Xylose release from ND-BSKP and ND-BHKP increased by 47 % and 57 %, respectively, highlighting the potential of PcaLOOLs to enhance hemicellulose recovery. Scanning electron microscopy and fiber image analysis revealed fibrillation and curlation of ND-BSKP after PcaLOOL treatment, consistent with increasing enzyme accessibility to targeted substrates.

AB - Microbial expansin-related proteins, including loosenins, can disrupt cellulose networks and increase enzyme accessibility to cellulosic substrates. Herein, four loosenins from Phanerochaete carnosa (PcaLOOLs), and a PcaLOOL fused to a family 63 carbohydrate-binding module, were compared for ability to boost the cellulolytic deconstruction of steam pretreated softwood (SSW) and kraft pulps from softwood (ND-BSKP) and hardwood (ND-BHKP). Amending the Cellic® CTec-2 cellulase cocktail with PcaLOOLs increased reducing products from SSW by up to 40 %, corresponding to 28 % higher glucose yield. Amending Cellic® CTec-2 with PcaLOOLs also increased the release of glucose from ND-BSKP and ND-BHKP by 82 % and 28 %, respectively. Xylose release from ND-BSKP and ND-BHKP increased by 47 % and 57 %, respectively, highlighting the potential of PcaLOOLs to enhance hemicellulose recovery. Scanning electron microscopy and fiber image analysis revealed fibrillation and curlation of ND-BSKP after PcaLOOL treatment, consistent with increasing enzyme accessibility to targeted substrates.

KW - Bioprocessing

KW - Carbohydrate-active enzymes

KW - Lignocellulose

KW - Loosenins

KW - Microbial expansins

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U2 - 10.1016/j.biortech.2023.130188

DO - 10.1016/j.biortech.2023.130188

M3 - Article

C2 - 38104665

AN - SCOPUS:85181020399

SN - 0960-8524

VL - 394

JO - Bioresource Technology

JF - Bioresource Technology

M1 - 130188

ER -

Fungal loosenin-like proteins boost the cellulolytic enzyme conversion of pretreated wood fiber and cellulosic pulps

Abstract

Keywords

UN SDGs

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BioUPGRADE: Biocatalytic upgrading of natural biopolymers for reassembly as multipurpose materials

Bioeconomy Research Infrastructure

Cite this

Fungal loosenin-like proteins boost the cellulolytic enzyme conversion of pretreated wood fiber and cellulosic pulps

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Projects

BioUPGRADE: Biocatalytic upgrading of natural biopolymers for reassembly as multipurpose materials

Equipment

Bioeconomy Research Infrastructure

Cite this