Functional Liquid-Fluid Interfaces Based onHydrophobin Proteins: - An Experimental Study for Medical Applications

Research output: ThesisDoctoral ThesisCollection of Articles

Abstract

Interfaces are everywhere around us. Any direct interaction occurs at the interface. This thesis explored the potential of functional interfaces to solve various medical application challenges. The four publications presented in this thesis highlight the different ways in which functional interfaces can be utilized to address these challenges. Publication I focused on the relationship between gravity, viscoelasticity, and the shape of water droplets coated with HFBI hydrophobin proteins. By studying the self-organization of hydrophobins at the air-water interface, it was found that a rigid layer is formed at a critical concentration, which affects the droplet morphology. This finding has significant implications for engineering and biomedical applications, as it provides a pathway for controlling the shape of droplets in various systems. Publication II presented a novel antibody extraction method using advanced materials and functional interfaces. By dividing an oil-based ferrofluid into daughter droplets under an external magnetic field, the surface area of liquid-liquid interfaces is increased, allowing for the functionalization and application of these interfaces as a substrate for antibody extraction. Publication III focused on the formation and characterization of protein-coated gas bubbles. By employing a micropipette aspiration technique, the mechanical properties of these bubbles were assessed, and a sealing parameter (Q) was determined to evaluate their gas permeability. These well-characterized bubbles have promising potential as ultrasound-enhanced contrast agents in various biomedical fields. They can be utilized for imaging purposes and targeted drug delivery, opening up new possibilities for medical diagnostics and therapies. Publication IV explored the utilization of hydrophobin protein functionalized bubbles to develop an advanced ultrasound molecular imaging probe. By functionalizing bubbles with a moiety part at their interface, they can attach to specific antigens and reveal diseased cells, such as cancer cells. This innovative approach holds great promise for improving the accuracy and sensitivity of molecular imaging techniques, enabling early detection and precise targeting of diseases. Overall, the findings presented in this thesis demonstrate the immense potential of functional interfaces in solving various medical application challenges. They provide valuable insights into the design and development of novel materials and techniques that can improve diagnostics, therapeutics, and imaging in the biomedical field.
Translated title of the contributionHydrofobiini Proteiineihin perustuvat funktionaaliset neste-fluidi-rajapinnat: Kokeellinen tutkimus lääketieteellisiin sovelluksiin
Original languageEnglish
QualificationDoctor's degree
Awarding Institution
  • Aalto University
Supervisors/Advisors
  • Ras, Robin, Supervising Professor
  • Paananen, Arja, Thesis Advisor, External person
  • Joensuu, Jussi, Thesis Advisor, External person
Publisher
Print ISBNs978-952-64-1814-8
Electronic ISBNs978-952-64-1815-5
Publication statusPublished - 2024
MoE publication typeG5 Doctoral dissertation (article)

Keywords

  • functional interfaces
  • proteins
  • bubbles
  • droplets
  • molecular imaging

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