From vitreoscilla hemoglobin (VHb) to a novel class of growth stimulating hemoglobin proteins

Pauli T. Kallio, Alexander D. Frey, James E. Bailey

Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

Abstract

Hemoglobins are a specific group of oxygen-binding heme proteins that have been identified in a wide range of distantly related organisms such as bacteria, yeast, mammals, and plants. The homodimeric hemoglobin of Vitreoscilla (VHb) is the best characterized bacterial hemoglobin consisting of two identical 15.8 kDa subunits. The expression of VHb is up-regulated by oxygen limitation (hypoxia), but despite of intensive research activities, its physiological functions have not yet been entirely elucidated in Vitreoscilla nor in heterologous microorganisms. Nonetheless, heterologous expression of VHb has been used to alleviate physiologically unfavorable effects of oxygen limitation, to improve growth properties and productivity of various microorganisms, plants and mammalian cells producing industrially important enzymes and compounds. We summarize the effects of VHb-expression on productivity and its putative biochemical function in Escherichia coli. In addition, we describe recent significant improvements in hemoglobin technology.

Original languageEnglish
Title of host publicationRecombinant Protein Production with Prokaryotic and Eukaryotic Cells
Subtitle of host publicationA Comparative View on Host Physiology
EditorsOW Merten, D Mattanovich, C Lang, G Larsson, P Neubauer, D Porro, P Postma, JT DeMattos, JA Cole
Pages75-87
Number of pages13
ISBN (Electronic)978-94-015-9749-4
Publication statusPublished - 2001
MoE publication typeA4 Article in a conference publication
EventConference of the Microbial Physiology Section of the European Federation of Biotechnology - Semmering, Austria
Duration: 5 Oct 20008 Oct 2000

Conference

ConferenceConference of the Microbial Physiology Section of the European Federation of Biotechnology
Country/TerritoryAustria
CitySemmering
Period05/10/200008/10/2000

Keywords

  • Vitreoscilla hemoglobin (VHb)
  • Alcaligenes eutrophus flavohemoglobin (FHP)
  • fusion proteins
  • VHb-mutants
  • microaerobic growth
  • Escherichia coli
  • two-dimensional Nuclear Magnetic Resonance spectroscopy (2D NMR)
  • fluxome
  • RECOMBINANT ESCHERICHIA-COLI
  • CENTRAL CARBON METABOLISM
  • INTRACELLULAR EXPRESSION
  • BACTERIAL HEMOGLOBIN
  • SACCHAROPOLYSPORA-ERYTHRAEA
  • MICROAEROBIC BIOPROCESSES
  • HETEROLOGOUS EXPRESSION
  • ALCALIGENES-EUTROPHUS
  • INDUSTRIAL STRAIN
  • ENERGY-METABOLISM

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