Fermentative production of extracellular amylase from novel amylase producer, Tuber maculatum mycelium, and its characterization

Dattatray Bedade*, Jan Deska, Sandip Bankar, Samir Bejar, Rekha Singhal, Salem Shamekh

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

8 Citations (Scopus)


Truffles are symbiotic hypogeous edible fungi (form of mushroom) that form filamentous mycelia in their initial phase of the growth cycle as well as a symbiotic association with host plant roots. In the present study, Tuber maculatum mycelia were isolated and tested for extracellular amylase production at different pH on solid agar medium. Furthermore, the mycelium was subjected to submerged fermentation for amylase production under different culture conditions such as variable carbon sources and their concentrations, initial medium pH, and incubation time. The optimized conditions after the experiments included soluble starch (0.5% w/v), initial medium pH of 7.0, and incubation time of 7 days, at room temperature (22 ± 2 °C) under static conditions which resulted in 1.41 U/mL of amylase. The amylase thus obtained was further characterized for its biocatalytic properties and found to have an optimum activity at pH 5.0 and a temperature of 50 °C. The enzyme showed good thermostability at 50 °C by retaining 98% of the maximal activity after 100 min of incubation. The amylase activity was marginally enhanced in presence of Cu2+ and Na+ and slightly reduced by K+, Ca2+, Fe2+, Mg2+, Co2+, Zn2+, and Mn2+ ions at 1 mM concentration.

Original languageEnglish
Pages (from-to)549-555
Number of pages7
JournalPreparative Biochemistry and Biotechnology
Issue number6
Publication statusPublished - 3 Jul 2018
MoE publication typeA1 Journal article-refereed


  • Amylase
  • biochemical characterization
  • starch
  • truffle
  • Tuber maculatum


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