Extremely low frequency electromagnetic stimulation alters osteoblast actin filament morphology

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Abstract

The effect of 50 Hz 1 mT sinusoidal electromagnetic field stimulation (EMF) on osteoblast-like MC3T3-E1 cell morphology was studied 24h after single exposure and after 3 weeks of daily stimulation. Scanning electron microscopy (SEM) revealed differences in F-actin-based filopodia and lamellipodia extent between stimulated and control cell cultures. As actin-based structures have been implicated in Ca2+ storage and signaling, these findings suggest that low EMF energies may be able to perturb Ca2+ signaling through affecting the morphology of these extensions. Furthermore, 3 week SEM observations confirm prior findings of EMF-enhanced differentiation of osteoblast-like cells as evidenced by improved spreading and mineralization of stimulated cells.

Details

Original languageEnglish
Title of host publicationIFMBE Proceedings
EditorsTomaz Jarm, Peter Kramar
Publication statusPublished - 2016
MoE publication typeA4 Article in a conference publication
EventWorld Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food and Environmental Technologies - Portoroz, Slovenia
Duration: 6 Sep 201510 Sep 2015
Conference number: 1

Publication series

NameIFMBE proceedings
PublisherInternational Federation for Medical Biological Engineering
Volume53
ISSN (Print)1680-0737
ISSN (Electronic)1433-9277

Conference

ConferenceWorld Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food and Environmental Technologies
CountrySlovenia
CityPortoroz
Period06/09/201510/09/2015

    Research areas

  • F-actin, Microvilli, Osteoblast morphology, Pulsed electromagnetic fields, SEM

ID: 1536208