Extremely low frequency electromagnetic stimulation alters osteoblast actin filament morphology

A.-M. Bique; T. Keskinen; Mervi Paulasto-Kröckel

doi:10.1007/978-981-287-817-5_34

Extremely low frequency electromagnetic stimulation alters osteoblast actin filament morphology

A.-M. Bique, T. Keskinen, Mervi Paulasto-Kröckel

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

Abstract

The effect of 50 Hz 1 mT sinusoidal electromagnetic field stimulation (EMF) on osteoblast-like MC3T3-E1 cell morphology was studied 24h after single exposure and after 3 weeks of daily stimulation. Scanning electron microscopy (SEM) revealed differences in F-actin-based filopodia and lamellipodia extent between stimulated and control cell cultures. As actin-based structures have been implicated in Ca2+ storage and signaling, these findings suggest that low EMF energies may be able to perturb Ca2+ signaling through affecting the morphology of these extensions. Furthermore, 3 week SEM observations confirm prior findings of EMF-enhanced differentiation of osteoblast-like cells as evidenced by improved spreading and mineralization of stimulated cells.

Original language	English
Title of host publication	IFMBE Proceedings
Editors	Tomaz Jarm, Peter Kramar
Publisher	Springer
Pages	155-158
Number of pages	4
Volume	53
ISBN (Electronic)	9789812878175
ISBN (Print)	9789812878168
DOIs	https://doi.org/10.1007/978-981-287-817-5_34
Publication status	Published - 2016
MoE publication type	A4 Conference publication
Event	World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food and Environmental Technologies - Portoroz, Slovenia Duration: 6 Sept 2015 → 10 Sept 2015 Conference number: 1

Publication series

Name	IFMBE proceedings
Publisher	International Federation for Medical Biological Engineering
Volume	53
ISSN (Print)	1680-0737
ISSN (Electronic)	1433-9277

Conference

Conference	World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food and Environmental Technologies
Country/Territory	Slovenia
City	Portoroz
Period	06/09/2015 → 10/09/2015

Keywords

F-actin
Microvilli
Osteoblast morphology
Pulsed electromagnetic fields
SEM

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10.1007/978-981-287-817-5_34

Cite this

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title = "Extremely low frequency electromagnetic stimulation alters osteoblast actin filament morphology",

abstract = "The effect of 50 Hz 1 mT sinusoidal electromagnetic field stimulation (EMF) on osteoblast-like MC3T3-E1 cell morphology was studied 24h after single exposure and after 3 weeks of daily stimulation. Scanning electron microscopy (SEM) revealed differences in F-actin-based filopodia and lamellipodia extent between stimulated and control cell cultures. As actin-based structures have been implicated in Ca2+ storage and signaling, these findings suggest that low EMF energies may be able to perturb Ca2+ signaling through affecting the morphology of these extensions. Furthermore, 3 week SEM observations confirm prior findings of EMF-enhanced differentiation of osteoblast-like cells as evidenced by improved spreading and mineralization of stimulated cells.",

keywords = "F-actin, Microvilli, Osteoblast morphology, Pulsed electromagnetic fields, SEM",

author = "A.-M. Bique and T. Keskinen and Mervi Paulasto-Kr{\"o}ckel",

year = "2016",

doi = "10.1007/978-981-287-817-5_34",

language = "English",

isbn = "9789812878168",

volume = "53",

series = "IFMBE proceedings",

publisher = "Springer",

pages = "155--158",

editor = "Tomaz Jarm and Peter Kramar",

booktitle = "IFMBE Proceedings",

address = "Germany",

note = "World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food and Environmental Technologies ; Conference date: 06-09-2015 Through 10-09-2015",

}

Bique, A-M, Keskinen, T & Paulasto-Kröckel, M 2016, Extremely low frequency electromagnetic stimulation alters osteoblast actin filament morphology. in T Jarm & P Kramar (eds), IFMBE Proceedings. vol. 53, IFMBE proceedings, vol. 53, Springer, pp. 155-158, World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food and Environmental Technologies, Portoroz, Slovenia, 06/09/2015. https://doi.org/10.1007/978-981-287-817-5_34

Extremely low frequency electromagnetic stimulation alters osteoblast actin filament morphology. / Bique, A.-M.; Keskinen, T.; Paulasto-Kröckel, Mervi.
IFMBE Proceedings. ed. / Tomaz Jarm; Peter Kramar. Vol. 53 Springer, 2016. p. 155-158 (IFMBE proceedings; Vol. 53).

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

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AU - Keskinen, T.

AU - Paulasto-Kröckel, Mervi

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Y1 - 2016

N2 - The effect of 50 Hz 1 mT sinusoidal electromagnetic field stimulation (EMF) on osteoblast-like MC3T3-E1 cell morphology was studied 24h after single exposure and after 3 weeks of daily stimulation. Scanning electron microscopy (SEM) revealed differences in F-actin-based filopodia and lamellipodia extent between stimulated and control cell cultures. As actin-based structures have been implicated in Ca2+ storage and signaling, these findings suggest that low EMF energies may be able to perturb Ca2+ signaling through affecting the morphology of these extensions. Furthermore, 3 week SEM observations confirm prior findings of EMF-enhanced differentiation of osteoblast-like cells as evidenced by improved spreading and mineralization of stimulated cells.

AB - The effect of 50 Hz 1 mT sinusoidal electromagnetic field stimulation (EMF) on osteoblast-like MC3T3-E1 cell morphology was studied 24h after single exposure and after 3 weeks of daily stimulation. Scanning electron microscopy (SEM) revealed differences in F-actin-based filopodia and lamellipodia extent between stimulated and control cell cultures. As actin-based structures have been implicated in Ca2+ storage and signaling, these findings suggest that low EMF energies may be able to perturb Ca2+ signaling through affecting the morphology of these extensions. Furthermore, 3 week SEM observations confirm prior findings of EMF-enhanced differentiation of osteoblast-like cells as evidenced by improved spreading and mineralization of stimulated cells.

KW - F-actin

KW - Microvilli

KW - Osteoblast morphology

KW - Pulsed electromagnetic fields

KW - SEM

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ER -

Extremely low frequency electromagnetic stimulation alters osteoblast actin filament morphology

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