TY - JOUR
T1 - Expression of a fungal hydrophobin in the Saccharomyces cerevisiae cell wall
T2 - Effect on cell surface properties and immobilization
AU - Nakari-Setälä, Tiina
AU - Azeredo, Joana
AU - Henriques, Mariana
AU - Oliveira, Rosário
AU - Teixeira, José
AU - Linder, Markus
AU - Penttilä, Merja
PY - 2002
Y1 - 2002
N2 - The aim of this work was to modify the cell surface properties of Saccharomyces cerevisiae by expression of the HFBI hydrophobin of the filamentous fungus Trichoderma reesei on the yeast cell surface. The second aim was to study the immobilization capacity of the modified cells. Fusion to the Flo1p flocculin was used to target the HFBI moiety to the cell wall. Determination of cell surface characteristics with contact angle and zeta potential measurements indicated that HFBI-producing cells are more apolar and slightly less negatively charged than the parent cells. Adsorption of the yeast cells to different commercial supports was studied. A twofold increase in the binding affinity of the hydrophobin-producing yeast to hydrophobic silicone-based materials was observed, while no improvement in the interaction with hydrophilic carriers could be seen compared to that of the parent cells. Hydrophobic interactions between the yeast cells and the support are suggested to play a major role in attachment. Also, a slight increase in the initial adsorption rate of the hydrophobin yeast was observed. Furthermore, due to the engineered cell surface, hydrophobin-producing yeast cells were efficiently separated in an aqueous two-phase system by using a nonionic polyoxyethylene detergent, C12-18EO5..
AB - The aim of this work was to modify the cell surface properties of Saccharomyces cerevisiae by expression of the HFBI hydrophobin of the filamentous fungus Trichoderma reesei on the yeast cell surface. The second aim was to study the immobilization capacity of the modified cells. Fusion to the Flo1p flocculin was used to target the HFBI moiety to the cell wall. Determination of cell surface characteristics with contact angle and zeta potential measurements indicated that HFBI-producing cells are more apolar and slightly less negatively charged than the parent cells. Adsorption of the yeast cells to different commercial supports was studied. A twofold increase in the binding affinity of the hydrophobin-producing yeast to hydrophobic silicone-based materials was observed, while no improvement in the interaction with hydrophilic carriers could be seen compared to that of the parent cells. Hydrophobic interactions between the yeast cells and the support are suggested to play a major role in attachment. Also, a slight increase in the initial adsorption rate of the hydrophobin yeast was observed. Furthermore, due to the engineered cell surface, hydrophobin-producing yeast cells were efficiently separated in an aqueous two-phase system by using a nonionic polyoxyethylene detergent, C12-18EO5..
UR - http://www.scopus.com/inward/record.url?scp=0036301038&partnerID=8YFLogxK
U2 - 10.1128/AEM.68.7.3385-3391.2002
DO - 10.1128/AEM.68.7.3385-3391.2002
M3 - Article
C2 - 12089019
AN - SCOPUS:0036301038
SN - 0099-2240
VL - 68
SP - 3385
EP - 3391
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 7
ER -