Expression of a fungal cellobiohydrolase in insect cells

I von Ossowski, T Teeri, N Kalkkinen, C OkerBlom

Research output: Contribution to journalArticleScientificpeer-review

Abstract

The gene for Trichoderma reesei cellobiohydrolase I (CBHI) was expressed with a recombinant baculovirus and high levels of secreted protein were produced in Spodoptera frugiperda and Trichoplusia ni insect cells. Electrophoretic analysis indicated that the recombinant CBHI (rCBHI) was similar in apparent molecular weight to the native form and immunoblotting with anti-CBHI monoclonal antibodies confirmed its identity. The rCBHI was easily purified by affinity and hydrophobic interaction chromatography and demonstrated enzymatic activity on soluble substrate. (C) 1997 Academic Press.

Original languageEnglish
Pages (from-to)25-29
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume233
Issue number1
DOIs
Publication statusPublished - 7 Apr 1997
MoE publication typeA1 Journal article-refereed

Keywords

  • TRICHODERMA-REESEI QM-9414
  • CELLULOSE-BINDING DOMAINS
  • POLYACRYLAMIDE GELS
  • BACULOVIRUS
  • PROTEINS
  • SYSTEM
  • CORE

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