Expression of a fungal cellobiohydrolase in insect cells

I von Ossowski; T Teeri; N Kalkkinen; C Oker-Blom

doi:10.1006/bbrc.1997.6391

Expression of a fungal cellobiohydrolase in insect cells

I von Ossowski, T Teeri, N Kalkkinen, C Oker-Blom

Not published at Aalto University

Research output: Contribution to journal › Article › Scientific › peer-review

Abstract

The gene for Trichoderma reesei cellobiohydrolase I (CBHI) was expressed with a recombinant baculovirus and high levels of secreted protein were produced in Spodoptera frugiperda and Trichoplusia ni insect cells. Electrophoretic analysis indicated that the recombinant CBHI (rCBHI) was similar in apparent molecular weight to the native form and immunoblotting with anti-CBHI monoclonal antibodies confirmed its identity. The rCBHI was easily purified by affinity and hydrophobic interaction chromatography and demonstrated enzymatic activity on soluble substrate. (C) 1997 Academic Press.

Original language	English
Pages (from-to)	25-29
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	233
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1997.6391
Publication status	Published - 7 Apr 1997
MoE publication type	A1 Journal article-refereed

Keywords

TRICHODERMA-REESEI QM-9414
CELLULOSE-BINDING DOMAINS
POLYACRYLAMIDE GELS
BACULOVIRUS
PROTEINS
SYSTEM
CORE

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title = "Expression of a fungal cellobiohydrolase in insect cells",

abstract = "The gene for Trichoderma reesei cellobiohydrolase I (CBHI) was expressed with a recombinant baculovirus and high levels of secreted protein were produced in Spodoptera frugiperda and Trichoplusia ni insect cells. Electrophoretic analysis indicated that the recombinant CBHI (rCBHI) was similar in apparent molecular weight to the native form and immunoblotting with anti-CBHI monoclonal antibodies confirmed its identity. The rCBHI was easily purified by affinity and hydrophobic interaction chromatography and demonstrated enzymatic activity on soluble substrate. (C) 1997 Academic Press.",

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author = "{von Ossowski}, I and T Teeri and N Kalkkinen and C Oker-Blom",

year = "1997",

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doi = "10.1006/bbrc.1997.6391",

language = "English",

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T1 - Expression of a fungal cellobiohydrolase in insect cells

AU - von Ossowski, I

AU - Teeri, T

AU - Kalkkinen, N

AU - Oker-Blom, C

PY - 1997/4/7

Y1 - 1997/4/7

N2 - The gene for Trichoderma reesei cellobiohydrolase I (CBHI) was expressed with a recombinant baculovirus and high levels of secreted protein were produced in Spodoptera frugiperda and Trichoplusia ni insect cells. Electrophoretic analysis indicated that the recombinant CBHI (rCBHI) was similar in apparent molecular weight to the native form and immunoblotting with anti-CBHI monoclonal antibodies confirmed its identity. The rCBHI was easily purified by affinity and hydrophobic interaction chromatography and demonstrated enzymatic activity on soluble substrate. (C) 1997 Academic Press.

AB - The gene for Trichoderma reesei cellobiohydrolase I (CBHI) was expressed with a recombinant baculovirus and high levels of secreted protein were produced in Spodoptera frugiperda and Trichoplusia ni insect cells. Electrophoretic analysis indicated that the recombinant CBHI (rCBHI) was similar in apparent molecular weight to the native form and immunoblotting with anti-CBHI monoclonal antibodies confirmed its identity. The rCBHI was easily purified by affinity and hydrophobic interaction chromatography and demonstrated enzymatic activity on soluble substrate. (C) 1997 Academic Press.

KW - TRICHODERMA-REESEI QM-9414

KW - CELLULOSE-BINDING DOMAINS

KW - POLYACRYLAMIDE GELS

KW - BACULOVIRUS

KW - PROTEINS

KW - SYSTEM

KW - CORE

U2 - 10.1006/bbrc.1997.6391

DO - 10.1006/bbrc.1997.6391

M3 - Article

SN - 0006-291X

VL - 233

SP - 25

EP - 29

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Expression of a fungal cellobiohydrolase in insect cells

Abstract

Keywords

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