Exploring the complexity of xylitol production in the fungal cell factory Aspergillus niger

Astrid Müller, Jiali Meng, Robin Kuijpers, Miia R. Mäkelä, Ronald P. de Vries*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

17 Downloads (Pure)

Abstract

Production of xylitol from agricultural by-products offers a promising approach for the circular bioeconomy. This study investigates the roles of transcription factors XlnR and CreA in xylitol production from wheat bran in Aspergillus niger by generating strains with a constitutively active XlnR (XlnRc, V756F mutation) and/or deletion of creA, in a previously generated xylitol-producing strain. The XlnRc mutation increased the initial rate of xylitol production but lowered the overall accumulation. Deletion of creA in this strain significantly improved both the onset and rate of xylitol production, indicating an inhibitory role of CreA in the PCP. These results demonstrate the complexity of metabolic engineering to generate fungal cell factories for valuable biochemicals, such as xylitol, as not only metabolic but also multiple gene regulation aspects need to be considered.

Original languageEnglish
Article number110550
Number of pages6
JournalEnzyme and Microbial Technology
Volume183
Early online date26 Nov 2024
DOIs
Publication statusE-pub ahead of print - 26 Nov 2024
MoE publication typeA1 Journal article-refereed

Keywords

  • Aspergillus niger
  • Circular economy
  • Fungal cell factory
  • Metabolic engineering
  • Pentose catabolic pathway
  • Xylitol

Fingerprint

Dive into the research topics of 'Exploring the complexity of xylitol production in the fungal cell factory Aspergillus niger'. Together they form a unique fingerprint.

Cite this