Evidence that the gene YLR070c of Saccharomyces cerevisiae encodes a xylitol dehydrogenase

Peter Richard; Mervi H. Toivari; Merja Penttilä

doi:10.1016/S0014-5793(99)01016-9

Evidence that the gene YLR070c of Saccharomyces cerevisiae encodes a xylitol dehydrogenase

Peter Richard^*, Mervi H. Toivari, Merja Penttilä

^*Corresponding author for this work

Not published at Aalto University

VTT Technical Research Centre of Finland

Research output: Contribution to journal › Article › Scientific › peer-review

86 Citations (Scopus)

Abstract

The open reading frame YLR070c of Saccharomyces cerevisiae has high sequence similarity to S. cerevisiae sorbitol dehydrogenase and to xylitol dehydrogenase of Pichia stipitis. Overexpression of this open reading frame in S, cerevisiae resulted in xylitol dehydrogenase activity. The enzyme is specific for NADH, The following Michaelis constants mere estimated: D-xylulose, 1.1 mM; NADH, 240 μM (at pH 7.0); xylitol, 25 mM; NAD, 100 μM (at pH 9.0), Xylitol dehydrogenase activity with the same kinetic properties can also be induced by xylose in mild type S, cerevisiae cells. (C) 1999 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	135-138
Number of pages	4
Journal	FEBS Letters
Volume	457
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(99)01016-9
Publication status	Published - 20 Aug 1999
MoE publication type	A1 Journal article-refereed

Keywords

xylitol dehydrogenase
xylose metabolism
D-xylulose
enzyme kinetics
Saccharomyces cerevisiae
XYLOSE FERMENTATION
PICHIA-STIPITIS
TRANSFORMATION
XYLULOKINASE
PURIFICATION
PATHWAY
GLUCOSE
CLONING
DNA

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title = "Evidence that the gene YLR070c of Saccharomyces cerevisiae encodes a xylitol dehydrogenase",

abstract = "The open reading frame YLR070c of Saccharomyces cerevisiae has high sequence similarity to S. cerevisiae sorbitol dehydrogenase and to xylitol dehydrogenase of Pichia stipitis. Overexpression of this open reading frame in S, cerevisiae resulted in xylitol dehydrogenase activity. The enzyme is specific for NADH, The following Michaelis constants mere estimated: D-xylulose, 1.1 mM; NADH, 240 μM (at pH 7.0); xylitol, 25 mM; NAD, 100 μM (at pH 9.0), Xylitol dehydrogenase activity with the same kinetic properties can also be induced by xylose in mild type S, cerevisiae cells. (C) 1999 Federation of European Biochemical Societies.",

keywords = "xylitol dehydrogenase, xylose metabolism, D-xylulose, enzyme kinetics, Saccharomyces cerevisiae, XYLOSE FERMENTATION, PICHIA-STIPITIS, TRANSFORMATION, XYLULOKINASE, PURIFICATION, PATHWAY, GLUCOSE, CLONING, DNA",

author = "Peter Richard and Toivari, \{Mervi H.\} and Merja Penttil{\"a}",

year = "1999",

month = aug,

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doi = "10.1016/S0014-5793(99)01016-9",

language = "English",

volume = "457",

pages = "135--138",

journal = "FEBS Letters",

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TY - JOUR

T1 - Evidence that the gene YLR070c of Saccharomyces cerevisiae encodes a xylitol dehydrogenase

AU - Richard, Peter

AU - Toivari, Mervi H.

AU - Penttilä, Merja

PY - 1999/8/20

Y1 - 1999/8/20

N2 - The open reading frame YLR070c of Saccharomyces cerevisiae has high sequence similarity to S. cerevisiae sorbitol dehydrogenase and to xylitol dehydrogenase of Pichia stipitis. Overexpression of this open reading frame in S, cerevisiae resulted in xylitol dehydrogenase activity. The enzyme is specific for NADH, The following Michaelis constants mere estimated: D-xylulose, 1.1 mM; NADH, 240 μM (at pH 7.0); xylitol, 25 mM; NAD, 100 μM (at pH 9.0), Xylitol dehydrogenase activity with the same kinetic properties can also be induced by xylose in mild type S, cerevisiae cells. (C) 1999 Federation of European Biochemical Societies.

AB - The open reading frame YLR070c of Saccharomyces cerevisiae has high sequence similarity to S. cerevisiae sorbitol dehydrogenase and to xylitol dehydrogenase of Pichia stipitis. Overexpression of this open reading frame in S, cerevisiae resulted in xylitol dehydrogenase activity. The enzyme is specific for NADH, The following Michaelis constants mere estimated: D-xylulose, 1.1 mM; NADH, 240 μM (at pH 7.0); xylitol, 25 mM; NAD, 100 μM (at pH 9.0), Xylitol dehydrogenase activity with the same kinetic properties can also be induced by xylose in mild type S, cerevisiae cells. (C) 1999 Federation of European Biochemical Societies.

KW - xylitol dehydrogenase

KW - xylose metabolism

KW - D-xylulose

KW - enzyme kinetics

KW - Saccharomyces cerevisiae

KW - XYLOSE FERMENTATION

KW - PICHIA-STIPITIS

KW - TRANSFORMATION

KW - XYLULOKINASE

KW - PURIFICATION

KW - PATHWAY

KW - GLUCOSE

KW - CLONING

KW - DNA

UR - https://www.scopus.com/pages/publications/0032769768

U2 - 10.1016/S0014-5793(99)01016-9

DO - 10.1016/S0014-5793(99)01016-9

M3 - Article

SN - 0014-5793

VL - 457

SP - 135

EP - 138

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Evidence that the gene YLR070c of Saccharomyces cerevisiae encodes a xylitol dehydrogenase

Abstract

Keywords

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