Estrogenic activity in Finnish municipal wastewater effluents

Pia Välitalo; Noora Perkola; Thomas-Benjamin Seiler; Markus Sillanpää; Jochen Kuckelkorn; Anna Mikola; Henner Hollert; Eija Schultz

doi:10.1016/j.watres.2015.10.056

Estrogenic activity in Finnish municipal wastewater effluents

Pia Välitalo, Noora Perkola, Thomas-Benjamin Seiler, Markus Sillanpää, Jochen Kuckelkorn, Anna Mikola, Henner Hollert, Eija Schultz

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Abstract

Effluents from wastewater treatment plants (WWTPs) are a major source of estrogenic compounds to the aquatic environment. In the present work, estrogenic activities of effluents from eight municipal WWTPs in Finland were studied. The main objectives of the study were to quantify the concentrations of selected estrogenic compounds, to evaluate their contribution to estrogenic potency and to test the feasibility of the commercial bioassays for wastewater analysis. The effluent samples were analyzed by two in vitro tests, i.e. ER alpha-CALUX (R) and ELISA-E2, and by liquid chromatography mass spectrometry for six estrogenic compounds: estrone (El), 17 beta-estradiol (E2), estriol (E3), 17 alpha-ethinylestradiol (EE2), 17 alpha-estradiol and bisphenol A (BPA). Estrogenic effects were found in all of the effluent samples with both of the bioassays. The concentrations measured with ELISA-E2 (8.6-61.6 ng/L) were clearly higher but exhibited a similar pattern than those with chemical analysis (E2

Original language	English
Pages (from-to)	740-749
Number of pages	10
Journal	Water Research
Volume	88
DOIs	https://doi.org/10.1016/j.watres.2015.10.056
Publication status	Published - 1 Jan 2016
MoE publication type	A1 Journal article-refereed

Keywords

Wastewater effluent
Estrogenic activity
In vitro
Chemical analysis
ENDOCRINE DISRUPTING COMPOUNDS
TREATMENT-PLANT EFFLUENTS
TANDEM MASS-SPECTROMETRY
SOLID-PHASE EXTRACTION
REPORTER GENE ASSAY
PERFORMANCE LIQUID-CHROMATOGRAPHY
ENVIRONMENTAL RISK-ASSESSMENT
MULTIPLE HORMONAL ACTIVITIES
SEWAGE-TREATMENT PLANTS
IN-VITRO BIOASSAYS

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title = "Estrogenic activity in Finnish municipal wastewater effluents",

abstract = "Effluents from wastewater treatment plants (WWTPs) are a major source of estrogenic compounds to the aquatic environment. In the present work, estrogenic activities of effluents from eight municipal WWTPs in Finland were studied. The main objectives of the study were to quantify the concentrations of selected estrogenic compounds, to evaluate their contribution to estrogenic potency and to test the feasibility of the commercial bioassays for wastewater analysis. The effluent samples were analyzed by two in vitro tests, i.e. ER alpha-CALUX (R) and ELISA-E2, and by liquid chromatography mass spectrometry for six estrogenic compounds: estrone (El), 17 beta-estradiol (E2), estriol (E3), 17 alpha-ethinylestradiol (EE2), 17 alpha-estradiol and bisphenol A (BPA). Estrogenic effects were found in all of the effluent samples with both of the bioassays. The concentrations measured with ELISA-E2 (8.6-61.6 ng/L) were clearly higher but exhibited a similar pattern than those with chemical analysis (E2",

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AU - Sillanpää, Markus

AU - Kuckelkorn, Jochen

AU - Mikola, Anna

AU - Hollert, Henner

AU - Schultz, Eija

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Estrogenic activity in Finnish municipal wastewater effluents

Abstract

Keywords

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