Abstract
The production of both homologous and heterologous proteins with the cellulolytic filamentous fungus Trichoderma reesei is described. Biotechnically important improvements in the production of cellulolytic enzymes have been obtained by genetic engineering methodology to construct strains secreting novel mixtures of cellulases. These improvements have been achieved by gene inactivation and promoter changes. The strong and highly inducible promoter of the gene encoding the major cellulase, cellobiohydrolase I (CBHI) has also been used for the production of eukaryotic heterologous proteins in Trichoderma. The expression and secretion of active calf chymosin is described in detail.
Original language | English |
---|---|
Pages (from-to) | 35-49 |
Number of pages | 15 |
Journal | Journal of Biotechnology |
Volume | 17 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 1991 |
MoE publication type | A1 Journal article-refereed |
Keywords
- ABBREVIATIONS
- ELISA
- ENZYME LINKED IMMUNOSORBENT ASSAY
- PAGE
- POLYACRYLAMIDE GEL ELECTROPHORESIS
- PMSF
- PHENYLMETHYLSULFONYL FLUORIDE
- SDS
- SODIUM DODECYL SULFATE
- TCA
- TRICHLOROACETIC ACID
- MOLECULAR-CLONING
- SACCHAROMYCES-CEREVISIAE
- CELLOBIOHYDROLASE-I
- NUCLEOTIDE-SEQUENCE
- BOVINE CHYMOSIN
- ENDOGLUCANASE-I
- EXPRESSION
- GENE
- CELLULASE
- SECRETION