Abstract
Three phase partitioning, a technique which has been explored for protein separation was evaluated for extraction of turmeric oleoresin. The process consists of simultaneous addition of t-butanol and ammonium sulphate to the aqueous slurry of turmeric powder. The extraction of oleoresin was optimized with respect to the concentration of ammonium sulphate loading and the ratio of t-butanol to slurry. Pretreatment of the slurries with a commercial enzyme preparation of α-amylase and/or glucoamylase followed by three phase partitioning was carried out. The extraction time with this technique is lower as compared to conventional acetone extraction.
| Original language | English |
|---|---|
| Pages (from-to) | 423-426 |
| Number of pages | 4 |
| Journal | Process Biochemistry |
| Volume | 46 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2011 |
| MoE publication type | A1 Journal article-refereed |
Keywords
- α-Amylase
- Enzyme assisted three phase partitioning
- Extraction
- Glucoamylase
- Turmeric oleoresin